Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31992 | 5' | -51.7 | NC_006949.1 | + | 7288 | 0.66 | 0.853292 |
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Target: 5'- cGGCAGCGGCACGacgcCAacagccauaccaaccAGCGGagaaaugaugUCCUg -3' miRNA: 3'- -CCGUCGCUGUGCaa--GU---------------UCGUCa---------AGGG- -5' |
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| 31992 | 5' | -51.7 | NC_006949.1 | + | 10415 | 0.67 | 0.812771 |
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Target: 5'- cGGCAG-GACGgcugcUGUUCAGGcCGGgagccaUCCCg -3' miRNA: 3'- -CCGUCgCUGU-----GCAAGUUC-GUCa-----AGGG- -5' |
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| 31992 | 5' | -51.7 | NC_006949.1 | + | 32003 | 0.68 | 0.782848 |
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Target: 5'- cGGUAGCGACAUuaUCAGGUcguAGUUUUUu -3' miRNA: 3'- -CCGUCGCUGUGcaAGUUCG---UCAAGGG- -5' |
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| 31992 | 5' | -51.7 | NC_006949.1 | + | 5930 | 0.68 | 0.762045 |
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Target: 5'- cGGCGGUGAUACGggUuucggucuuguaGAGCGauUUCCCg -3' miRNA: 3'- -CCGUCGCUGUGCaaG------------UUCGUc-AAGGG- -5' |
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| 31992 | 5' | -51.7 | NC_006949.1 | + | 15743 | 0.74 | 0.443569 |
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Target: 5'- uGGCAaaGAUACGUUCucagaAAGCAGUUagCCCa -3' miRNA: 3'- -CCGUcgCUGUGCAAG-----UUCGUCAA--GGG- -5' |
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| 31992 | 5' | -51.7 | NC_006949.1 | + | 4965 | 1.15 | 0.00084 |
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Target: 5'- uGGCAGCGACACGUUCAAGCAGUUCCCu -3' miRNA: 3'- -CCGUCGCUGUGCAAGUUCGUCAAGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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