Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
31995 | 5' | -51.6 | NC_006949.1 | + | 33507 | 0.66 | 0.831788 |
Target: 5'- uGCCGG-GCUggGCCAUgcCAGCAucuuGCAa -3' miRNA: 3'- -CGGCUgCGA--CGGUAaaGUCGUuu--CGU- -5' |
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31995 | 5' | -51.6 | NC_006949.1 | + | 14745 | 0.67 | 0.802497 |
Target: 5'- uGCCaaugGugGCUGCCAacUCGGUAu-GCGa -3' miRNA: 3'- -CGG----CugCGACGGUaaAGUCGUuuCGU- -5' |
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31995 | 5' | -51.6 | NC_006949.1 | + | 26747 | 0.67 | 0.792323 |
Target: 5'- uGCCGAU-UUGCUuUUUCAGCAcccGGCAa -3' miRNA: 3'- -CGGCUGcGACGGuAAAGUCGUu--UCGU- -5' |
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31995 | 5' | -51.6 | NC_006949.1 | + | 45283 | 0.67 | 0.768243 |
Target: 5'- cGCUGACGCUucGUCAUgUCGGUAAuugucgcguucgccAGCGu -3' miRNA: 3'- -CGGCUGCGA--CGGUAaAGUCGUU--------------UCGU- -5' |
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31995 | 5' | -51.6 | NC_006949.1 | + | 19566 | 0.68 | 0.749909 |
Target: 5'- gGCCGGCGUgGUCGU---GGUGAAGCAc -3' miRNA: 3'- -CGGCUGCGaCGGUAaagUCGUUUCGU- -5' |
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31995 | 5' | -51.6 | NC_006949.1 | + | 26608 | 0.68 | 0.738942 |
Target: 5'- gGCUGGCGUU-CCgAUUUCGGUAuuGCAg -3' miRNA: 3'- -CGGCUGCGAcGG-UAAAGUCGUuuCGU- -5' |
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31995 | 5' | -51.6 | NC_006949.1 | + | 7015 | 0.68 | 0.704246 |
Target: 5'- cCCGAagaaagcaaacagUGCUGUCAUaUCGGCAGGGUc -3' miRNA: 3'- cGGCU-------------GCGACGGUAaAGUCGUUUCGu -5' |
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31995 | 5' | -51.6 | NC_006949.1 | + | 14383 | 0.68 | 0.694016 |
Target: 5'- uGCCGcCGCUGCCG----AGCAAcGCu -3' miRNA: 3'- -CGGCuGCGACGGUaaagUCGUUuCGu -5' |
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31995 | 5' | -51.6 | NC_006949.1 | + | 14542 | 0.69 | 0.682586 |
Target: 5'- cGCUGGUGCUGCCAUgcUAGCGGccAGUg -3' miRNA: 3'- -CGGCUGCGACGGUAaaGUCGUU--UCGu -5' |
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31995 | 5' | -51.6 | NC_006949.1 | + | 28440 | 0.7 | 0.624888 |
Target: 5'- aCCGACGCUGgCGgaaCGGCGuaGAGCu -3' miRNA: 3'- cGGCUGCGACgGUaaaGUCGU--UUCGu -5' |
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31995 | 5' | -51.6 | NC_006949.1 | + | 18732 | 0.7 | 0.578779 |
Target: 5'- gGCUGACGUgUGCCAguggCAGUAucGCGg -3' miRNA: 3'- -CGGCUGCG-ACGGUaaa-GUCGUuuCGU- -5' |
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31995 | 5' | -51.6 | NC_006949.1 | + | 10393 | 1.12 | 0.001063 |
Target: 5'- aGCCGACGCUGCCAUUUCAGCAAAGCAa -3' miRNA: 3'- -CGGCUGCGACGGUAAAGUCGUUUCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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