Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31997 | 3' | -58.6 | NC_006949.1 | + | 9232 | 0.66 | 0.447848 |
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Target: 5'- cUCGAU-GCaGCCAGuCGGCGGCaucacCGCc -3' miRNA: 3'- uAGUUGuCG-CGGUC-GCCGCCGau---GCG- -5' |
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| 31997 | 3' | -58.6 | NC_006949.1 | + | 16292 | 0.66 | 0.447848 |
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Target: 5'- -aCGGCGGCcggaGCCcaagcagaugcGGCGGCGGUUGCc- -3' miRNA: 3'- uaGUUGUCG----CGG-----------UCGCCGCCGAUGcg -5' |
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| 31997 | 3' | -58.6 | NC_006949.1 | + | 14461 | 0.67 | 0.409473 |
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Target: 5'- -gCGGCAGauaagcguugcuCGgCAGCGGCGGCauucaucGCGCu -3' miRNA: 3'- uaGUUGUC------------GCgGUCGCCGCCGa------UGCG- -5' |
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| 31997 | 3' | -58.6 | NC_006949.1 | + | 31295 | 0.67 | 0.409473 |
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Target: 5'- ---cGCGGCGUUGGCGGCGGauaACGa -3' miRNA: 3'- uaguUGUCGCGGUCGCCGCCga-UGCg -5' |
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| 31997 | 3' | -58.6 | NC_006949.1 | + | 6306 | 0.67 | 0.409473 |
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Target: 5'- ------cGCGCCugcAGCGGCGGCagugccaACGCg -3' miRNA: 3'- uaguuguCGCGG---UCGCCGCCGa------UGCG- -5' |
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| 31997 | 3' | -58.6 | NC_006949.1 | + | 5955 | 0.7 | 0.235789 |
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Target: 5'- -aCGGCAGUGCCuggcaguaguucuGCGGCGGUgaUACGg -3' miRNA: 3'- uaGUUGUCGCGGu------------CGCCGCCG--AUGCg -5' |
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| 31997 | 3' | -58.6 | NC_006949.1 | + | 7798 | 0.71 | 0.208823 |
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Target: 5'- aAUUAGCAGCGCCGuuGCgGGCGGgUAaugUGCa -3' miRNA: 3'- -UAGUUGUCGCGGU--CG-CCGCCgAU---GCG- -5' |
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| 31997 | 3' | -58.6 | NC_006949.1 | + | 14381 | 0.76 | 0.093434 |
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Target: 5'- cUCGucCAGCGCCcGUucuuuugcgacGGCGGCUGCGCg -3' miRNA: 3'- uAGUu-GUCGCGGuCG-----------CCGCCGAUGCG- -5' |
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| 31997 | 3' | -58.6 | NC_006949.1 | + | 14490 | 1.1 | 0.000282 |
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Target: 5'- uAUCAACAGCGCCAGCGGCGGCUACGCg -3' miRNA: 3'- -UAGUUGUCGCGGUCGCCGCCGAUGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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