miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
31998 3' -48.5 NC_006949.1 + 46632 0.66 0.93656
Target:  5'- -cGCAGCCcuuaAUGGGagcuccUUCUGCGUGAGUg -3'
miRNA:   3'- guCGUUGG----UAUCU------GAGACGUAUUCGu -5'
31998 3' -48.5 NC_006949.1 + 36910 0.66 0.930722
Target:  5'- gCAGUAGCUA-AGGCUUUGgCAUuAGCGa -3'
miRNA:   3'- -GUCGUUGGUaUCUGAGAC-GUAuUCGU- -5'
31998 3' -48.5 NC_006949.1 + 40552 0.7 0.774365
Target:  5'- aCAGCAaugaACCGUGGAUUCaaaaGCAUuucuGGCAu -3'
miRNA:   3'- -GUCGU----UGGUAUCUGAGa---CGUAu---UCGU- -5'
31998 3' -48.5 NC_006949.1 + 43028 0.71 0.718016
Target:  5'- cCAGaagGCgAUGGAUuaUCUGCAUGAGCGa -3'
miRNA:   3'- -GUCgu-UGgUAUCUG--AGACGUAUUCGU- -5'
31998 3' -48.5 NC_006949.1 + 9423 0.72 0.68289
Target:  5'- cCAGCGcuuACCAgcGACUCgGCAUGAuGCGc -3'
miRNA:   3'- -GUCGU---UGGUauCUGAGaCGUAUU-CGU- -5'
31998 3' -48.5 NC_006949.1 + 16367 1.1 0.002932
Target:  5'- gCAGCAACCAUAGACUCUGCAUAAGCAg -3'
miRNA:   3'- -GUCGUUGGUAUCUGAGACGUAUUCGU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.