Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 31999 | 3' | -56.8 | NC_006949.1 | + | 3506 | 0.66 | 0.543309 |
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Target: 5'- cCAGaCCAUGCUG-GGCAGCGGUg-- -3' miRNA: 3'- cGUUaGGUACGGCgUCGUCGCCGuca -5' |
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| 31999 | 3' | -56.8 | NC_006949.1 | + | 19839 | 0.66 | 0.51095 |
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Target: 5'- cGCuAUCacUGCCGCuGCuGCGGCAu- -3' miRNA: 3'- -CGuUAGguACGGCGuCGuCGCCGUca -5' |
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| 31999 | 3' | -56.8 | NC_006949.1 | + | 16246 | 0.67 | 0.469123 |
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Target: 5'- -uGGUUgAUGCCaGUgauGCAGCGGCAGc -3' miRNA: 3'- cgUUAGgUACGG-CGu--CGUCGCCGUCa -5' |
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| 31999 | 3' | -56.8 | NC_006949.1 | + | 14459 | 0.67 | 0.448875 |
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Target: 5'- gGCAGauaagCguUGCUcgGCAGCGGCGGCAu- -3' miRNA: 3'- -CGUUa----GguACGG--CGUCGUCGCCGUca -5' |
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| 31999 | 3' | -56.8 | NC_006949.1 | + | 14217 | 0.7 | 0.32221 |
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Target: 5'- gGCuacCCAUaacaGCAGCAGCGGCGGUc -3' miRNA: 3'- -CGuuaGGUAcgg-CGUCGUCGCCGUCA- -5' |
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| 31999 | 3' | -56.8 | NC_006949.1 | + | 6319 | 0.73 | 0.205699 |
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Target: 5'- --uAUCCuuuuguuacgcGCCuGCAGCGGCGGCAGUg -3' miRNA: 3'- cguUAGGua---------CGG-CGUCGUCGCCGUCA- -5' |
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| 31999 | 3' | -56.8 | NC_006949.1 | + | 19918 | 1.1 | 0.000386 |
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Target: 5'- cGCAAUCCAUGCCGCAGCAGCGGCAGUg -3' miRNA: 3'- -CGUUAGGUACGGCGUCGUCGCCGUCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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