Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32002 | 3' | -57.4 | NC_006949.1 | + | 21963 | 0.66 | 0.571961 |
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Target: 5'- ----cCCGAAUGUGGAgaaCCUGCGCAu- -3' miRNA: 3'- auuguGGCUUGCGCCU---GGGCGCGUcu -5' |
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| 32002 | 3' | -57.4 | NC_006949.1 | + | 23562 | 0.66 | 0.571961 |
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Target: 5'- cAGCACUGGauaaugAUGCGGuuCCGC-CAGAg -3' miRNA: 3'- aUUGUGGCU------UGCGCCugGGCGcGUCU- -5' |
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| 32002 | 3' | -57.4 | NC_006949.1 | + | 18572 | 0.66 | 0.539628 |
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Target: 5'- uUAACuuuCCGAACgguaauccugauGCGGACCCGaCaCAGGa -3' miRNA: 3'- -AUUGu--GGCUUG------------CGCCUGGGC-GcGUCU- -5' |
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| 32002 | 3' | -57.4 | NC_006949.1 | + | 497 | 0.67 | 0.507937 |
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Target: 5'- -cACGgUGGACGUGGAUCUGCuaAGAc -3' miRNA: 3'- auUGUgGCUUGCGCCUGGGCGcgUCU- -5' |
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| 32002 | 3' | -57.4 | NC_006949.1 | + | 5594 | 0.67 | 0.477061 |
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Target: 5'- aAACcCUGAcCGCuaacACCCGCGCAGAa -3' miRNA: 3'- aUUGuGGCUuGCGcc--UGGGCGCGUCU- -5' |
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| 32002 | 3' | -57.4 | NC_006949.1 | + | 14322 | 0.68 | 0.399705 |
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Target: 5'- gGGCGCUGGACGa-GAUCCGCcuuGCGGAg -3' miRNA: 3'- aUUGUGGCUUGCgcCUGGGCG---CGUCU- -5' |
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| 32002 | 3' | -57.4 | NC_006949.1 | + | 25035 | 0.69 | 0.39061 |
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Target: 5'- cAGCGCCGGAuugUGuCGGACCUGUGUAa- -3' miRNA: 3'- aUUGUGGCUU---GC-GCCUGGGCGCGUcu -5' |
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| 32002 | 3' | -57.4 | NC_006949.1 | + | 4179 | 0.69 | 0.37283 |
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Target: 5'- aAGCGCCGGGCGaacaGGAUagCGCGCAc- -3' miRNA: 3'- aUUGUGGCUUGCg---CCUGg-GCGCGUcu -5' |
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| 32002 | 3' | -57.4 | NC_006949.1 | + | 13382 | 0.73 | 0.203546 |
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Target: 5'- cGGCAUgGAAgGUGGAUucaaCCGCGCAGAu -3' miRNA: 3'- aUUGUGgCUUgCGCCUG----GGCGCGUCU- -5' |
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| 32002 | 3' | -57.4 | NC_006949.1 | + | 28547 | 1.07 | 0.000647 |
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Target: 5'- aUAACACCGAACGCGGACCCGCGCAGAg -3' miRNA: 3'- -AUUGUGGCUUGCGCCUGGGCGCGUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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