Results 1 - 20 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
32003 | 3' | -53.9 | NC_006949.1 | + | 28439 | 0.66 | 0.733437 |
Target: 5'- uACCGACGCUGGCggaaCGGcgUAG-AGCu -3' miRNA: 3'- -UGGUUGUGACCGaug-GUCa-GUCgUCG- -5' |
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32003 | 3' | -53.9 | NC_006949.1 | + | 33436 | 0.66 | 0.722468 |
Target: 5'- --aGAUGCUGGCauggcCCAGccCGGCAGCg -3' miRNA: 3'- uggUUGUGACCGau---GGUCa-GUCGUCG- -5' |
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32003 | 3' | -53.9 | NC_006949.1 | + | 24544 | 0.66 | 0.704722 |
Target: 5'- uACC-GCGCUGGCUAUCcauuuaacuggcaauGGgUAGCAGUu -3' miRNA: 3'- -UGGuUGUGACCGAUGG---------------UCaGUCGUCG- -5' |
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32003 | 3' | -53.9 | NC_006949.1 | + | 14481 | 0.66 | 0.677754 |
Target: 5'- cGCCAGCGgcGGCUACgcgagcggCAGauaagcguugcUCGGCAGCg -3' miRNA: 3'- -UGGUUGUgaCCGAUG--------GUC-----------AGUCGUCG- -5' |
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32003 | 3' | -53.9 | NC_006949.1 | + | 18943 | 0.67 | 0.670964 |
Target: 5'- gAUUGGCAUUGGUU-CCGGUCgcggauaacggaaacGGCAGCc -3' miRNA: 3'- -UGGUUGUGACCGAuGGUCAG---------------UCGUCG- -5' |
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32003 | 3' | -53.9 | NC_006949.1 | + | 38569 | 0.67 | 0.666429 |
Target: 5'- aACCAGCcgUGGUUauaACCAGcgacCAGUAGCg -3' miRNA: 3'- -UGGUUGugACCGA---UGGUCa---GUCGUCG- -5' |
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32003 | 3' | -53.9 | NC_006949.1 | + | 5147 | 0.68 | 0.586822 |
Target: 5'- gGCCAACGCccuGCU-CCGGagUAGCGGCu -3' miRNA: 3'- -UGGUUGUGac-CGAuGGUCa-GUCGUCG- -5' |
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32003 | 3' | -53.9 | NC_006949.1 | + | 9217 | 0.68 | 0.575532 |
Target: 5'- aACCGaauGCAUcaucucgauGCaGCCAGUCGGCGGCa -3' miRNA: 3'- -UGGU---UGUGac-------CGaUGGUCAGUCGUCG- -5' |
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32003 | 3' | -53.9 | NC_006949.1 | + | 29017 | 0.68 | 0.564291 |
Target: 5'- uACCAGCcaguGC-GGUgaugACCucGUCGGCAGCa -3' miRNA: 3'- -UGGUUG----UGaCCGa---UGGu-CAGUCGUCG- -5' |
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32003 | 3' | -53.9 | NC_006949.1 | + | 14621 | 0.68 | 0.564291 |
Target: 5'- aAUC-GCACUGGCcGCUAGcauGGCAGCa -3' miRNA: 3'- -UGGuUGUGACCGaUGGUCag-UCGUCG- -5' |
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32003 | 3' | -53.9 | NC_006949.1 | + | 24035 | 0.69 | 0.518907 |
Target: 5'- aGCCAuaGCACcacaGGCUGCUGGUUuuaugguGGCAGUa -3' miRNA: 3'- -UGGU--UGUGa---CCGAUGGUCAG-------UCGUCG- -5' |
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32003 | 3' | -53.9 | NC_006949.1 | + | 14215 | 0.7 | 0.486632 |
Target: 5'- gGCC-GCAgUuGCUGCaagaaauCAGUCAGCAGCa -3' miRNA: 3'- -UGGuUGUgAcCGAUG-------GUCAGUCGUCG- -5' |
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32003 | 3' | -53.9 | NC_006949.1 | + | 8484 | 0.7 | 0.46669 |
Target: 5'- cGCCGugGugucaccuCUGGCgaugUACCAGUCucgcuGCGGCa -3' miRNA: 3'- -UGGUugU--------GACCG----AUGGUCAGu----CGUCG- -5' |
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32003 | 3' | -53.9 | NC_006949.1 | + | 14745 | 0.73 | 0.334653 |
Target: 5'- uGCCAAUGgUGGCUGCCAacUCGGUAuGCg -3' miRNA: 3'- -UGGUUGUgACCGAUGGUc-AGUCGU-CG- -5' |
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32003 | 3' | -53.9 | NC_006949.1 | + | 14229 | 0.76 | 0.20243 |
Target: 5'- cACCAACAUaacGGCUACCcauaacAG-CAGCAGCg -3' miRNA: 3'- -UGGUUGUGa--CCGAUGG------UCaGUCGUCG- -5' |
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32003 | 3' | -53.9 | NC_006949.1 | + | 34944 | 0.87 | 0.039126 |
Target: 5'- aACCAACACUGGCgACCAG-UAGCGGCn -3' miRNA: 3'- -UGGUUGUGACCGaUGGUCaGUCGUCG- -5' |
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32003 | 3' | -53.9 | NC_006949.1 | + | 35106 | 0.9 | 0.02061 |
Target: 5'- gAUCAugACUGGCgACCAGUCAGCAGCn -3' miRNA: 3'- -UGGUugUGACCGaUGGUCAGUCGUCG- -5' |
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32003 | 3' | -53.9 | NC_006949.1 | + | 34891 | 0.93 | 0.013826 |
Target: 5'- aACCAACACUGGCaACUGGUCAGCGGCn -3' miRNA: 3'- -UGGUUGUGACCGaUGGUCAGUCGUCG- -5' |
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32003 | 3' | -53.9 | NC_006949.1 | + | 35052 | 0.94 | 0.011146 |
Target: 5'- aACCAACACUGGCaACUGGUCAGCAGCn -3' miRNA: 3'- -UGGUUGUGACCGaUGGUCAGUCGUCG- -5' |
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32003 | 3' | -53.9 | NC_006949.1 | + | 34998 | 0.96 | 0.008189 |
Target: 5'- aACCAACACUGGCaACUGGUCAGCGGCa -3' miRNA: 3'- -UGGUUGUGACCGaUGGUCAGUCGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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