Results 1 - 19 of 19 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
32004 | 3' | -56 | NC_006949.1 | + | 45466 | 0.66 | 0.617543 |
Target: 5'- cUGG--GGCGGgAugCAuuauccACGCUGGCGGc -3' miRNA: 3'- -ACCagUCGCCgUugGU------UGUGACCGCU- -5' |
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32004 | 3' | -56 | NC_006949.1 | + | 22332 | 0.66 | 0.60527 |
Target: 5'- aGGUCAGCagagaacaagccuGGCGuCCAgauaACACUGGaUGGu -3' miRNA: 3'- aCCAGUCG-------------CCGUuGGU----UGUGACC-GCU- -5' |
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32004 | 3' | -56 | NC_006949.1 | + | 28370 | 0.67 | 0.551115 |
Target: 5'- uUGGUUAGUaaaGaGCAGCCGGCGCcGGUa- -3' miRNA: 3'- -ACCAGUCG---C-CGUUGGUUGUGaCCGcu -5' |
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32004 | 3' | -56 | NC_006949.1 | + | 41408 | 0.67 | 0.529411 |
Target: 5'- cUGG-CAGCGGgAaauuACC--UACUGGCGAc -3' miRNA: 3'- -ACCaGUCGCCgU----UGGuuGUGACCGCU- -5' |
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32004 | 3' | -56 | NC_006949.1 | + | 15967 | 0.67 | 0.517608 |
Target: 5'- cGGUaCAGCGGCAuucuUCAGCGCaagagcauuaucgUGcGCGAu -3' miRNA: 3'- aCCA-GUCGCCGUu---GGUUGUG-------------AC-CGCU- -5' |
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32004 | 3' | -56 | NC_006949.1 | + | 23016 | 0.68 | 0.497476 |
Target: 5'- cUGGUCGGCGGCAACgAGaGCaGuaGAu -3' miRNA: 3'- -ACCAGUCGCCGUUGgUUgUGaCcgCU- -5' |
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32004 | 3' | -56 | NC_006949.1 | + | 24220 | 0.68 | 0.475644 |
Target: 5'- cGGUUGGUGGUguuucGGCCAGCAUUGuucucauGCGAg -3' miRNA: 3'- aCCAGUCGCCG-----UUGGUUGUGAC-------CGCU- -5' |
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32004 | 3' | -56 | NC_006949.1 | + | 18176 | 0.68 | 0.45631 |
Target: 5'- -cGUCAGUGGUGG--GACACUGGCGc -3' miRNA: 3'- acCAGUCGCCGUUggUUGUGACCGCu -5' |
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32004 | 3' | -56 | NC_006949.1 | + | 3404 | 0.69 | 0.446305 |
Target: 5'- aGGUCuacGCGGCGagACUGACAUcgGGCGu -3' miRNA: 3'- aCCAGu--CGCCGU--UGGUUGUGa-CCGCu -5' |
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32004 | 3' | -56 | NC_006949.1 | + | 34926 | 0.83 | 0.046384 |
Target: 5'- ---gUAGCGGCAACCAACACUGGCu- -3' miRNA: 3'- accaGUCGCCGUUGGUUGUGACCGcu -5' |
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32004 | 3' | -56 | NC_006949.1 | + | 34847 | 0.84 | 0.041187 |
Target: 5'- -aGUCAGCaGCAACCAACACUGGCu- -3' miRNA: 3'- acCAGUCGcCGUUGGUUGUGACCGcu -5' |
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32004 | 3' | -56 | NC_006949.1 | + | 34901 | 0.85 | 0.035487 |
Target: 5'- -aGUCAGCaGCAACCAACACUGGCa- -3' miRNA: 3'- acCAGUCGcCGUUGGUUGUGACCGcu -5' |
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32004 | 3' | -56 | NC_006949.1 | + | 35089 | 0.85 | 0.035487 |
Target: 5'- -aGUCAGCaGCAACCAACACUGGCa- -3' miRNA: 3'- acCAGUCGcCGUUGGUUGUGACCGcu -5' |
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32004 | 3' | -56 | NC_006949.1 | + | 35008 | 0.95 | 0.007196 |
Target: 5'- -aGUCAGCGGCAACCAACACUGGCa- -3' miRNA: 3'- acCAGUCGCCGUUGGUUGUGACCGcu -5' |
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32004 | 3' | -56 | NC_006949.1 | + | 35037 | 0.99 | 0.003373 |
Target: 5'- cUGGUCAGCaGCAACCAACACUGGCGAc -3' miRNA: 3'- -ACCAGUCGcCGUUGGUUGUGACCGCU- -5' |
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32004 | 3' | -56 | NC_006949.1 | + | 34876 | 1.02 | 0.00214 |
Target: 5'- cUGGUCAGCGGCAACCAACACUGGCu- -3' miRNA: 3'- -ACCAGUCGCCGUUGGUUGUGACCGcu -5' |
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32004 | 3' | -56 | NC_006949.1 | + | 34983 | 1.03 | 0.001839 |
Target: 5'- cUGGUCAGCGGCAACCAACACUGGCa- -3' miRNA: 3'- -ACCAGUCGCCGUUGGUUGUGACCGcu -5' |
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32004 | 3' | -56 | NC_006949.1 | + | 35064 | 1.03 | 0.001839 |
Target: 5'- cUGGUCAGCGGCAACCAACACUGGCa- -3' miRNA: 3'- -ACCAGUCGCCGUUGGUUGUGACCGcu -5' |
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32004 | 3' | -56 | NC_006949.1 | + | 34956 | 1.08 | 0.000675 |
Target: 5'- cUGGUCAGCGGCAACCAACACUGGCGAc -3' miRNA: 3'- -ACCAGUCGCCGUUGGUUGUGACCGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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