Results 1 - 20 of 22 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
32005 | 3' | -53.4 | NC_006949.1 | + | 14485 | 0.66 | 0.716581 |
Target: 5'- aCAGcgcCAGCGGCGGCU-ACGCgagcGGCAGa -3' miRNA: 3'- -GUCa--GUCGUCGUUGGuUGUGa---CCGUU- -5' |
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32005 | 3' | -53.4 | NC_006949.1 | + | 18176 | 0.66 | 0.716581 |
Target: 5'- -cGUCAGUGGUGG--GACACUGGCGc -3' miRNA: 3'- guCAGUCGUCGUUggUUGUGACCGUu -5' |
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32005 | 3' | -53.4 | NC_006949.1 | + | 6299 | 0.66 | 0.69383 |
Target: 5'- gCAG-CGGCGGCAguGCCAACGC--GCAu -3' miRNA: 3'- -GUCaGUCGUCGU--UGGUUGUGacCGUu -5' |
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32005 | 3' | -53.4 | NC_006949.1 | + | 22332 | 0.66 | 0.682349 |
Target: 5'- aGGUCAGCAGagAACaAGC-CUGGCGu -3' miRNA: 3'- gUCAGUCGUCg-UUGgUUGuGACCGUu -5' |
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32005 | 3' | -53.4 | NC_006949.1 | + | 14678 | 0.67 | 0.636015 |
Target: 5'- gAGUUGGCAGCcACCA---UUGGCAAg -3' miRNA: 3'- gUCAGUCGUCGuUGGUuguGACCGUU- -5' |
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32005 | 3' | -53.4 | NC_006949.1 | + | 45471 | 0.67 | 0.624391 |
Target: 5'- cCAGUCuggGGCGGgAugCAuuauccACGCUGGCGg -3' miRNA: 3'- -GUCAG---UCGUCgUugGU------UGUGACCGUu -5' |
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32005 | 3' | -53.4 | NC_006949.1 | + | 15766 | 0.68 | 0.555185 |
Target: 5'- gCAGUUAGCccaauGCAAUCAACuaUGGCAGa -3' miRNA: 3'- -GUCAGUCGu----CGUUGGUUGugACCGUU- -5' |
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32005 | 3' | -53.4 | NC_006949.1 | + | 11612 | 0.69 | 0.488391 |
Target: 5'- ---gCAGCAGCAucACUAACACUGGa-- -3' miRNA: 3'- gucaGUCGUCGU--UGGUUGUGACCguu -5' |
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32005 | 3' | -53.4 | NC_006949.1 | + | 15646 | 0.7 | 0.475473 |
Target: 5'- --cUCAGCAGCGGCCugcgcuuuaaguGCAUUGGCu- -3' miRNA: 3'- gucAGUCGUCGUUGGu-----------UGUGACCGuu -5' |
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32005 | 3' | -53.4 | NC_006949.1 | + | 14236 | 0.72 | 0.367895 |
Target: 5'- uCAGUCAGCAGCAGa-GAUGCaGGCGg -3' miRNA: 3'- -GUCAGUCGUCGUUggUUGUGaCCGUu -5' |
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32005 | 3' | -53.4 | NC_006949.1 | + | 28369 | 0.72 | 0.367895 |
Target: 5'- uGGUUAGUaaagAGCAGCCGGCGCcGGUAGu -3' miRNA: 3'- gUCAGUCG----UCGUUGGUUGUGaCCGUU- -5' |
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32005 | 3' | -53.4 | NC_006949.1 | + | 16163 | 0.74 | 0.269441 |
Target: 5'- gGGUCAgGCAGCuGCCGcugcauCACUGGCAu -3' miRNA: 3'- gUCAGU-CGUCGuUGGUu-----GUGACCGUu -5' |
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32005 | 3' | -53.4 | NC_006949.1 | + | 34929 | 0.87 | 0.033792 |
Target: 5'- cCAG-UAGCGGCAACCAACACUGGCu- -3' miRNA: 3'- -GUCaGUCGUCGUUGGUUGUGACCGuu -5' |
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32005 | 3' | -53.4 | NC_006949.1 | + | 34875 | 0.97 | 0.006028 |
Target: 5'- uGGUCAGCGGCAACCAACACUGGCu- -3' miRNA: 3'- gUCAGUCGUCGUUGGUUGUGACCGuu -5' |
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32005 | 3' | -53.4 | NC_006949.1 | + | 34955 | 1.01 | 0.003209 |
Target: 5'- uGGUCAGCGGCAACCAACACUGGCGAc -3' miRNA: 3'- gUCAGUCGUCGUUGGUUGUGACCGUU- -5' |
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32005 | 3' | -53.4 | NC_006949.1 | + | 34849 | 1.01 | 0.003013 |
Target: 5'- cCAGUCAGCAGCAACCAACACUGGCu- -3' miRNA: 3'- -GUCAGUCGUCGUUGGUUGUGACCGuu -5' |
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32005 | 3' | -53.4 | NC_006949.1 | + | 35036 | 1.01 | 0.003013 |
Target: 5'- uGGUCAGCAGCAACCAACACUGGCGAn -3' miRNA: 3'- gUCAGUCGUCGUUGGUUGUGACCGUU- -5' |
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32005 | 3' | -53.4 | NC_006949.1 | + | 34982 | 1.02 | 0.002919 |
Target: 5'- uGGUCAGCGGCAACCAACACUGGCAAc -3' miRNA: 3'- gUCAGUCGUCGUUGGUUGUGACCGUU- -5' |
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32005 | 3' | -53.4 | NC_006949.1 | + | 35063 | 1.02 | 0.002919 |
Target: 5'- uGGUCAGCGGCAACCAACACUGGCAAc -3' miRNA: 3'- gUCAGUCGUCGUUGGUUGUGACCGUU- -5' |
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32005 | 3' | -53.4 | NC_006949.1 | + | 35010 | 1.04 | 0.001818 |
Target: 5'- cCAGUCAGCGGCAACCAACACUGGCAAc -3' miRNA: 3'- -GUCAGUCGUCGUUGGUUGUGACCGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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