Results 1 - 20 of 21 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
32005 | 5' | -51.8 | NC_006949.1 | + | 37825 | 0.66 | 0.838806 |
Target: 5'- uGGCAAgCAGCACUuuaaaauGUcGCUuGGUCAGa -3' miRNA: 3'- -CCGUUgGUUGUGAc------CGuUGA-CCAGUC- -5' |
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32005 | 5' | -51.8 | NC_006949.1 | + | 10700 | 0.67 | 0.80031 |
Target: 5'- uGGCGGCCcguaaGCUGGUca-UGGUCAu -3' miRNA: 3'- -CCGUUGGuug--UGACCGuugACCAGUc -5' |
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32005 | 5' | -51.8 | NC_006949.1 | + | 16259 | 0.67 | 0.790178 |
Target: 5'- cGCAACCAugGgUaGcGCAGCugcuguUGGUCAGa -3' miRNA: 3'- cCGUUGGUugUgA-C-CGUUG------ACCAGUC- -5' |
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32005 | 5' | -51.8 | NC_006949.1 | + | 28966 | 0.67 | 0.779867 |
Target: 5'- uGGCGcguaUAGCGCUGGCAucGCUGGa--- -3' miRNA: 3'- -CCGUug--GUUGUGACCGU--UGACCaguc -5' |
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32005 | 5' | -51.8 | NC_006949.1 | + | 14682 | 0.67 | 0.758758 |
Target: 5'- uGGCAGCCAcCAUUGGCAAgUuaaGcCAGa -3' miRNA: 3'- -CCGUUGGUuGUGACCGUUgAc--CaGUC- -5' |
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32005 | 5' | -51.8 | NC_006949.1 | + | 34855 | 0.68 | 0.735989 |
Target: 5'- uGGUAGCCAGugUUGGUugccgcuGACUGGUCGc -3' miRNA: 3'- -CCGUUGGUUguGACCG-------UUGACCAGUc -5' |
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32005 | 5' | -51.8 | NC_006949.1 | + | 35110 | 0.68 | 0.703748 |
Target: 5'- aGCAgAUCAugACUGGCGACcaGUCAGc -3' miRNA: 3'- cCGU-UGGUugUGACCGUUGacCAGUC- -5' |
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32005 | 5' | -51.8 | NC_006949.1 | + | 33358 | 0.68 | 0.702623 |
Target: 5'- ---uACCAGCacgACUGGCAauuauugGCUGGUCAc -3' miRNA: 3'- ccguUGGUUG---UGACCGU-------UGACCAGUc -5' |
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32005 | 5' | -51.8 | NC_006949.1 | + | 10397 | 0.72 | 0.510075 |
Target: 5'- uGGCAGCCGACGCUGccauuucagcaaaGCAACgGG-CAu -3' miRNA: 3'- -CCGUUGGUUGUGAC-------------CGUUGaCCaGUc -5' |
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32005 | 5' | -51.8 | NC_006949.1 | + | 43668 | 0.72 | 0.478879 |
Target: 5'- ---uGCCAuuGCACUGGCAugcgUUGGUCAGa -3' miRNA: 3'- ccguUGGU--UGUGACCGUu---GACCAGUC- -5' |
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32005 | 5' | -51.8 | NC_006949.1 | + | 6125 | 0.76 | 0.310424 |
Target: 5'- uGGuCAAUUcACACUGGCAACUGGUg-- -3' miRNA: 3'- -CC-GUUGGuUGUGACCGUUGACCAguc -5' |
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32005 | 5' | -51.8 | NC_006949.1 | + | 34840 | 0.79 | 0.185712 |
Target: 5'- aGCAACCAACACUGGCuACcaGUCAGc -3' miRNA: 3'- cCGUUGGUUGUGACCGuUGacCAGUC- -5' |
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32005 | 5' | -51.8 | NC_006949.1 | + | 34948 | 0.81 | 0.156642 |
Target: 5'- cGGCAACCAACACUGGCGAC----CAGu -3' miRNA: 3'- -CCGUUGGUUGUGACCGUUGaccaGUC- -5' |
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32005 | 5' | -51.8 | NC_006949.1 | + | 34922 | 0.84 | 0.087232 |
Target: 5'- cGGCAACCAACACUGGCuACcaGUCAGc -3' miRNA: 3'- -CCGUUGGUUGUGACCGuUGacCAGUC- -5' |
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32005 | 5' | -51.8 | NC_006949.1 | + | 34868 | 0.84 | 0.087232 |
Target: 5'- cGGCAACCAACACUGGCuACcaGUCAGc -3' miRNA: 3'- -CCGUUGGUUGUGACCGuUGacCAGUC- -5' |
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32005 | 5' | -51.8 | NC_006949.1 | + | 35028 | 0.86 | 0.068641 |
Target: 5'- aGCAACCAACACUGGCGACcaGUCAGn -3' miRNA: 3'- cCGUUGGUUGUGACCGUUGacCAGUC- -5' |
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32005 | 5' | -51.8 | NC_006949.1 | + | 35082 | 1.05 | 0.003134 |
Target: 5'- aGCAACCAACACUGGCAACUGGUCAGn -3' miRNA: 3'- cCGUUGGUUGUGACCGUUGACCAGUC- -5' |
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32005 | 5' | -51.8 | NC_006949.1 | + | 34894 | 1.06 | 0.002767 |
Target: 5'- aGCAACCAACACUGGCAACUGGUCAGc -3' miRNA: 3'- cCGUUGGUUGUGACCGUUGACCAGUC- -5' |
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32005 | 5' | -51.8 | NC_006949.1 | + | 34975 | 1.11 | 0.00123 |
Target: 5'- cGGCAACCAACACUGGCAACUGGUCAGc -3' miRNA: 3'- -CCGUUGGUUGUGACCGUUGACCAGUC- -5' |
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32005 | 5' | -51.8 | NC_006949.1 | + | 35002 | 1.11 | 0.00123 |
Target: 5'- cGGCAACCAACACUGGCAACUGGUCAGc -3' miRNA: 3'- -CCGUUGGUUGUGACCGUUGACCAGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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