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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32007 | 5' | -51 | NC_006949.1 | + | 18808 | 0.66 | 0.878887 |
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Target: 5'- gCCGCGAUACuGCCAcuggCACAcGUCAGc -3' miRNA: 3'- aGGUGCUGUG-CGGUag--GUGUuUAGUU- -5' |
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| 32007 | 5' | -51 | NC_006949.1 | + | 19503 | 0.66 | 0.862198 |
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Target: 5'- aCCACGAcCACGCCggCCAUcuGAGcCGGa -3' miRNA: 3'- aGGUGCU-GUGCGGuaGGUG--UUUaGUU- -5' |
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| 32007 | 5' | -51 | NC_006949.1 | + | 35896 | 0.67 | 0.806043 |
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Target: 5'- cUCCACcACACGCCAgUCCAaaccAAUCu- -3' miRNA: 3'- -AGGUGcUGUGCGGU-AGGUgu--UUAGuu -5' |
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| 32007 | 5' | -51 | NC_006949.1 | + | 37297 | 0.68 | 0.76441 |
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Target: 5'- aUCaGCGACuuuACGCUGUCCACGAAUa-- -3' miRNA: 3'- -AGgUGCUG---UGCGGUAGGUGUUUAguu -5' |
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| 32007 | 5' | -51 | NC_006949.1 | + | 2418 | 0.68 | 0.75359 |
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Target: 5'- cCCAUacgGGCGCguGCCAUCCGCGcAUCGu -3' miRNA: 3'- aGGUG---CUGUG--CGGUAGGUGUuUAGUu -5' |
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| 32007 | 5' | -51 | NC_006949.1 | + | 38846 | 1.07 | 0.002543 |
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Target: 5'- uUCCACGACACGCCAUCCACAAAUCAAg -3' miRNA: 3'- -AGGUGCUGUGCGGUAGGUGUUUAGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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