miRNA display CGI


Results 1 - 4 of 4 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
32010 3' -56.4 NC_006949.1 + 6474 0.66 0.611352
Target:  5'- aCCGUcaggUGCUGCAacugcgugauCAGGAGGUUGGCa-- -3'
miRNA:   3'- -GGCA----ACGGCGU----------GUCCUUCAGUCGggu -5'
32010 3' -56.4 NC_006949.1 + 22362 0.68 0.452232
Target:  5'- gUCGUUGCCGUaaugcgcaugACGGGGaccaGGUCAGCa-- -3'
miRNA:   3'- -GGCAACGGCG----------UGUCCU----UCAGUCGggu -5'
32010 3' -56.4 NC_006949.1 + 15936 0.69 0.417329
Target:  5'- aCGcaUUGCCGCACaguGGGAaauuuuccgcaacagAGUCAGgCCAa -3'
miRNA:   3'- gGC--AACGGCGUG---UCCU---------------UCAGUCgGGU- -5'
32010 3' -56.4 NC_006949.1 + 45770 1.1 0.000497
Target:  5'- aCCGUUGCCGCACAGGAAGUCAGCCCAc -3'
miRNA:   3'- -GGCAACGGCGUGUCCUUCAGUCGGGU- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.