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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32010 | 5' | -49.6 | NC_006949.1 | + | 10744 | 0.66 | 0.938645 |
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Target: 5'- aGUCGGCACU--CUGGCuUUCUCuuucuGCGg -3' miRNA: 3'- -CGGUCGUGAuuGACUGuAGGAGu----UGC- -5' |
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| 32010 | 5' | -49.6 | NC_006949.1 | + | 13680 | 0.66 | 0.938103 |
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Target: 5'- aCCAGCGCUGaugaucuggcuaaGCUGACAaCCaUCAu-- -3' miRNA: 3'- cGGUCGUGAU-------------UGACUGUaGG-AGUugc -5' |
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| 32010 | 5' | -49.6 | NC_006949.1 | + | 24486 | 0.66 | 0.921042 |
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Target: 5'- aGCCAGCGCgguAUUG-CGUCUgaUUGGCGa -3' miRNA: 3'- -CGGUCGUGau-UGACuGUAGG--AGUUGC- -5' |
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| 32010 | 5' | -49.6 | NC_006949.1 | + | 19448 | 0.66 | 0.913894 |
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Target: 5'- cCCGGCGCUGACccuugccacgcaaUGAUucUCCUCGAa- -3' miRNA: 3'- cGGUCGUGAUUG-------------ACUGu-AGGAGUUgc -5' |
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| 32010 | 5' | -49.6 | NC_006949.1 | + | 45676 | 0.66 | 0.912554 |
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Target: 5'- aGUUAGUGCUGgcgagccucgguggGCUGACuUCCUguGCGg -3' miRNA: 3'- -CGGUCGUGAU--------------UGACUGuAGGAguUGC- -5' |
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| 32010 | 5' | -49.6 | NC_006949.1 | + | 24838 | 0.67 | 0.899952 |
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Target: 5'- -gCGGCACUGauuuuucaacugaACUGGCAUCCggUGACu -3' miRNA: 3'- cgGUCGUGAU-------------UGACUGUAGGa-GUUGc -5' |
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| 32010 | 5' | -49.6 | NC_006949.1 | + | 1691 | 0.68 | 0.86937 |
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Target: 5'- cCCAGCAgUggUUGuucuUAUCCUCGAUa -3' miRNA: 3'- cGGUCGUgAuuGACu---GUAGGAGUUGc -5' |
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| 32010 | 5' | -49.6 | NC_006949.1 | + | 11691 | 0.68 | 0.860839 |
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Target: 5'- uUCAGCACcGACUGGCAgg-UCAAUGg -3' miRNA: 3'- cGGUCGUGaUUGACUGUaggAGUUGC- -5' |
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| 32010 | 5' | -49.6 | NC_006949.1 | + | 37417 | 0.7 | 0.751786 |
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Target: 5'- uGCCAGUucuGCcAACUccuCAUCCUCGACa -3' miRNA: 3'- -CGGUCG---UGaUUGAcu-GUAGGAGUUGc -5' |
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| 32010 | 5' | -49.6 | NC_006949.1 | + | 45735 | 1.13 | 0.001569 |
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Target: 5'- cGCCAGCACUAACUGACAUCCUCAACGg -3' miRNA: 3'- -CGGUCGUGAUUGACUGUAGGAGUUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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