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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
32014 | 3' | -49.2 | NC_006949.1 | + | 25049 | 0.67 | 0.937761 |
Target: 5'- aGCGcUGAggcacugCGugGACUgaauuacgacaaACAGAACUGGc -3' miRNA: 3'- -CGCuACUa------GCugCUGA------------UGUCUUGGCU- -5' |
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32014 | 3' | -49.2 | NC_006949.1 | + | 19900 | 0.67 | 0.92276 |
Target: 5'- aGCGGcagUGAuagcgccuUCGACuaccggcacaaacagGACUACAGAACCa- -3' miRNA: 3'- -CGCU---ACU--------AGCUG---------------CUGAUGUCUUGGcu -5' |
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32014 | 3' | -49.2 | NC_006949.1 | + | 33889 | 0.68 | 0.913846 |
Target: 5'- cGCGAUGGcaaagUGugGGCUAUGGuGCUGGc -3' miRNA: 3'- -CGCUACUa----GCugCUGAUGUCuUGGCU- -5' |
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32014 | 3' | -49.2 | NC_006949.1 | + | 2728 | 0.7 | 0.824594 |
Target: 5'- aCGAaaGGUCGugGGCUACAGAAgguuuCCGu -3' miRNA: 3'- cGCUa-CUAGCugCUGAUGUCUU-----GGCu -5' |
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32014 | 3' | -49.2 | NC_006949.1 | + | 9196 | 1.12 | 0.002595 |
Target: 5'- gGCGAUGAUCGACGACUACAGAACCGAa -3' miRNA: 3'- -CGCUACUAGCUGCUGAUGUCUUGGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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