Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
32018 | 5' | -53 | NC_006949.1 | + | 3294 | 0.66 | 0.782372 |
Target: 5'- --uGCGGCUGCUGUcuggaggUUCaCGUUguauguugcuguaggGUCCGc -3' miRNA: 3'- cauCGCCGACGGCA-------AAG-GCAA---------------UAGGC- -5' |
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32018 | 5' | -53 | NC_006949.1 | + | 965 | 0.68 | 0.647998 |
Target: 5'- gGUGGC-GCUGCCGUccuucUCCGgguUCCa -3' miRNA: 3'- -CAUCGcCGACGGCAa----AGGCaauAGGc -5' |
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32018 | 5' | -53 | NC_006949.1 | + | 7223 | 0.69 | 0.63554 |
Target: 5'- uUGGCGucgugccGCUGCCGcgUCCGUUA-CCu -3' miRNA: 3'- cAUCGC-------CGACGGCaaAGGCAAUaGGc -5' |
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32018 | 5' | -53 | NC_006949.1 | + | 33652 | 0.71 | 0.496832 |
Target: 5'- -aAGCGcGCUGCCGUUcaguggggaaucggUCGUUAUCUGu -3' miRNA: 3'- caUCGC-CGACGGCAAa-------------GGCAAUAGGC- -5' |
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32018 | 5' | -53 | NC_006949.1 | + | 18850 | 1.09 | 0.001478 |
Target: 5'- cGUAGCGGCUGCCGUUUCCGUUAUCCGc -3' miRNA: 3'- -CAUCGCCGACGGCAAAGGCAAUAGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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