Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32021 | 3' | -48.1 | NC_006949.1 | + | 37585 | 0.68 | 0.912193 |
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Target: 5'- aAUCCCUCAgguGAAUcAGGGugucGAAUCUCUu -3' miRNA: 3'- -UAGGGAGU---UUUAcUCCCu---UUUAGGGG- -5' |
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| 32021 | 3' | -48.1 | NC_006949.1 | + | 27260 | 0.69 | 0.890221 |
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Target: 5'- aAUCCCUgCAAAAUGcuGGAgccaGAAUCCUa -3' miRNA: 3'- -UAGGGA-GUUUUACucCCU----UUUAGGGg -5' |
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| 32021 | 3' | -48.1 | NC_006949.1 | + | 38025 | 0.72 | 0.744632 |
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Target: 5'- uUCCCUCAuuuUGAGGGAuAcgCCa- -3' miRNA: 3'- uAGGGAGUuuuACUCCCUuUuaGGgg -5' |
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| 32021 | 3' | -48.1 | NC_006949.1 | + | 38069 | 0.84 | 0.175238 |
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Target: 5'- cUCCCUCAAuuUGAGGGAuguuuuAUCCCUc -3' miRNA: 3'- uAGGGAGUUuuACUCCCUuu----UAGGGG- -5' |
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| 32021 | 3' | -48.1 | NC_006949.1 | + | 38004 | 0.89 | 0.087968 |
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Target: 5'- cAUCCCUCAAAuUGAGGGAGugcuAUCCCUc -3' miRNA: 3'- -UAGGGAGUUUuACUCCCUUu---UAGGGG- -5' |
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| 32021 | 3' | -48.1 | NC_006949.1 | + | 37960 | 1.11 | 0.00316 |
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Target: 5'- uAUCCCUCAAAAUGAGGGAAAAUCCCCu -3' miRNA: 3'- -UAGGGAGUUUUACUCCCUUUUAGGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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