Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32022 | 3' | -49.4 | NC_006949.1 | + | 34367 | 0.66 | 0.940014 |
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Target: 5'- uGCCCUucugaAUCACUucucgacagAAUUACCAGAAaauUACGa -3' miRNA: 3'- -CGGGA-----UAGUGG---------UUAGUGGUCUU---GUGU- -5' |
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| 32022 | 3' | -49.4 | NC_006949.1 | + | 38745 | 0.66 | 0.940014 |
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Target: 5'- cCCUUGUCGCCGuugacGUUACCGGcAACcCAc -3' miRNA: 3'- cGGGAUAGUGGU-----UAGUGGUC-UUGuGU- -5' |
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| 32022 | 3' | -49.4 | NC_006949.1 | + | 10200 | 0.66 | 0.93451 |
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Target: 5'- cGCUaUGUCGCgAAuuggcUCACCGGAACAg- -3' miRNA: 3'- -CGGgAUAGUGgUU-----AGUGGUCUUGUgu -5' |
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| 32022 | 3' | -49.4 | NC_006949.1 | + | 44163 | 0.68 | 0.879416 |
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Target: 5'- cGUCCUAUC-CC-GUCGCCAcGAAUgaGCAa -3' miRNA: 3'- -CGGGAUAGuGGuUAGUGGU-CUUG--UGU- -5' |
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| 32022 | 3' | -49.4 | NC_006949.1 | + | 3978 | 0.69 | 0.816314 |
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Target: 5'- uGCCCUGUC-CCuuUCucagcaGCCAGGGCGa- -3' miRNA: 3'- -CGGGAUAGuGGuuAG------UGGUCUUGUgu -5' |
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| 32022 | 3' | -49.4 | NC_006949.1 | + | 44051 | 1.12 | 0.001981 |
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Target: 5'- cGCCCUAUCACCAAUCACCAGAACACAu -3' miRNA: 3'- -CGGGAUAGUGGUUAGUGGUCUUGUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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