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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32023 | 3' | -53.7 | NC_006949.1 | + | 45680 | 0.66 | 0.744258 |
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Target: 5'- -aGUGCUGGCgAGCCUCggugGgCUGAc- -3' miRNA: 3'- ggCGCGAUUG-UCGGAGaa--CgGACUag -5' |
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| 32023 | 3' | -53.7 | NC_006949.1 | + | 28581 | 0.68 | 0.610068 |
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Target: 5'- uCCGCGCUAuCAGCCUUgcggcguuCCUGuagcUCa -3' miRNA: 3'- -GGCGCGAUuGUCGGAGaac-----GGACu---AG- -5' |
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| 32023 | 3' | -53.7 | NC_006949.1 | + | 14425 | 0.68 | 0.610068 |
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Target: 5'- aUCGCGCUAACcGCCgUC-UGCUUcAUCa -3' miRNA: 3'- -GGCGCGAUUGuCGG-AGaACGGAcUAG- -5' |
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| 32023 | 3' | -53.7 | NC_006949.1 | + | 2080 | 0.69 | 0.56503 |
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Target: 5'- -gGuCGCUAcgGCGGCCUUuuuuaUUGCCUGAa- -3' miRNA: 3'- ggC-GCGAU--UGUCGGAG-----AACGGACUag -5' |
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| 32023 | 3' | -53.7 | NC_006949.1 | + | 37412 | 0.69 | 0.531823 |
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Target: 5'- aCUGCGggAACAGUUUUgagGCCUGAUg -3' miRNA: 3'- -GGCGCgaUUGUCGGAGaa-CGGACUAg -5' |
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| 32023 | 3' | -53.7 | NC_006949.1 | + | 11747 | 0.78 | 0.177463 |
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Target: 5'- uCgGUGCUGaaaucacauGCAGCCUCUUGCCUcaacagGAUCu -3' miRNA: 3'- -GgCGCGAU---------UGUCGGAGAACGGA------CUAG- -5' |
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| 32023 | 3' | -53.7 | NC_006949.1 | + | 44174 | 1.12 | 0.00065 |
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Target: 5'- aCCGCGCUAACAGCCUCUUGCCUGAUCu -3' miRNA: 3'- -GGCGCGAUUGUCGGAGAACGGACUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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