Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32024 | 5' | -48.9 | NC_006949.1 | + | 20776 | 0.66 | 0.956724 |
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Target: 5'- uCUCCGCUauuGCGGGugcaGUUAUUg-ACCg -3' miRNA: 3'- -GAGGCGAgu-UGCCC----UAAUAAagUGGg -5' |
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| 32024 | 5' | -48.9 | NC_006949.1 | + | 46336 | 0.66 | 0.947103 |
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Target: 5'- aUCUGCUCAGCGguaagguaaucccGGAUUGcuucugUUUugCCg -3' miRNA: 3'- gAGGCGAGUUGC-------------CCUAAUa-----AAGugGG- -5' |
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| 32024 | 5' | -48.9 | NC_006949.1 | + | 38070 | 0.67 | 0.937267 |
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Target: 5'- aCUCC-CUCAAUuugaGGGAU-GUUUUAUCCc -3' miRNA: 3'- -GAGGcGAGUUG----CCCUAaUAAAGUGGG- -5' |
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| 32024 | 5' | -48.9 | NC_006949.1 | + | 42250 | 0.67 | 0.931651 |
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Target: 5'- gUCUGCUCAGUGGGAUgccgggcaUUACCg -3' miRNA: 3'- gAGGCGAGUUGCCCUAauaa----AGUGGg -5' |
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| 32024 | 5' | -48.9 | NC_006949.1 | + | 45384 | 0.67 | 0.925729 |
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Target: 5'- cCUCCGCcgcCAGCGuGGAUaAUgcauccCGCCCc -3' miRNA: 3'- -GAGGCGa--GUUGC-CCUAaUAaa----GUGGG- -5' |
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| 32024 | 5' | -48.9 | NC_006949.1 | + | 13250 | 0.7 | 0.831788 |
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Target: 5'- -cUCGCUcCGGCGGGGUUuuuuaUUGCCCg -3' miRNA: 3'- gaGGCGA-GUUGCCCUAAuaa--AGUGGG- -5' |
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| 32024 | 5' | -48.9 | NC_006949.1 | + | 12686 | 0.7 | 0.822241 |
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Target: 5'- -cCCGCUaCGGCGGGAUUug-UCGuuCCUg -3' miRNA: 3'- gaGGCGA-GUUGCCCUAAuaaAGU--GGG- -5' |
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| 32024 | 5' | -48.9 | NC_006949.1 | + | 45429 | 1.14 | 0.001994 |
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Target: 5'- aCUCCGCUCAACGGGAUUAUUUCACCCu -3' miRNA: 3'- -GAGGCGAGUUGCCCUAAUAAAGUGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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