miRNA display CGI


Results 1 - 6 of 6 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
32036 3' -51.8 NC_006953.1 + 3472 0.66 0.759202
Target:  5'- uGGaacCCGCgCAagaaAGGCUcuCCAGUUGACa -3'
miRNA:   3'- -CCgauGGCG-GU----UCCGAu-GGUUAACUG- -5'
32036 3' -51.8 NC_006953.1 + 17123 0.67 0.714599
Target:  5'- uGGCgaaaGgCGCCAAGGCaGCgGGUUcGGCu -3'
miRNA:   3'- -CCGa---UgGCGGUUCCGaUGgUUAA-CUG- -5'
32036 3' -51.8 NC_006953.1 + 28922 0.67 0.714599
Target:  5'- aGGCgacuuuuuCCGCgCAuucgcgcacGGGCUACCAGaggaUGACa -3'
miRNA:   3'- -CCGau------GGCG-GU---------UCCGAUGGUUa---ACUG- -5'
32036 3' -51.8 NC_006953.1 + 14223 0.67 0.713462
Target:  5'- uGGCUGCUGCCAcaguuccGGcGCUcCCAccgGGCu -3'
miRNA:   3'- -CCGAUGGCGGU-------UC-CGAuGGUuaaCUG- -5'
32036 3' -51.8 NC_006953.1 + 17227 0.72 0.402537
Target:  5'- cGCUGCUGCCGuGGUugcugUGCCAAUagUGGCu -3'
miRNA:   3'- cCGAUGGCGGUuCCG-----AUGGUUA--ACUG- -5'
32036 3' -51.8 NC_006953.1 + 18638 1.13 0.000611
Target:  5'- aGGCUACCGCCAAGGCUACCAAUUGACa -3'
miRNA:   3'- -CCGAUGGCGGUUCCGAUGGUUAACUG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.