Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32036 | 5' | -54 | NC_006953.1 | + | 17966 | 0.66 | 0.605689 |
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Target: 5'- uGAGGCUGaacUUGaCGCAGCCAacaaggccguagaaGCAGCa -3' miRNA: 3'- -CUUCGAU---GACgGCGUCGGUaa------------CGUCG- -5' |
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| 32036 | 5' | -54 | NC_006953.1 | + | 17227 | 0.67 | 0.558324 |
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Target: 5'- --cGCUGCUGCCGUGGU---UGCuGUg -3' miRNA: 3'- cuuCGAUGACGGCGUCGguaACGuCG- -5' |
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| 32036 | 5' | -54 | NC_006953.1 | + | 10296 | 0.67 | 0.524332 |
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Target: 5'- -cGGCUACcGuuGCAGgUacuaagAUUGCAGCg -3' miRNA: 3'- cuUCGAUGaCggCGUCgG------UAACGUCG- -5' |
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| 32036 | 5' | -54 | NC_006953.1 | + | 22030 | 0.68 | 0.469591 |
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Target: 5'- --cGCUAUcGCUGaaagcuCAGCCAaUGCAGCg -3' miRNA: 3'- cuuCGAUGaCGGC------GUCGGUaACGUCG- -5' |
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| 32036 | 5' | -54 | NC_006953.1 | + | 22158 | 0.7 | 0.373566 |
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Target: 5'- -uAGC-ACUGCCGCcugugucgggccauaAGCCuUUGUAGCc -3' miRNA: 3'- cuUCGaUGACGGCG---------------UCGGuAACGUCG- -5' |
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| 32036 | 5' | -54 | NC_006953.1 | + | 18603 | 1.11 | 0.000432 |
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Target: 5'- aGAAGCUACUGCCGCAGCCAUUGCAGCc -3' miRNA: 3'- -CUUCGAUGACGGCGUCGGUAACGUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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