Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32038 | 5' | -48 | NC_006953.1 | + | 7409 | 0.67 | 0.901326 |
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Target: 5'- gAGCUUGuAGCGcucGCUAAGGAGGaaGCa -3' miRNA: 3'- gUUGAACuUCGC---CGGUUCUUUUggCG- -5' |
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| 32038 | 5' | -48 | NC_006953.1 | + | 28434 | 0.67 | 0.901326 |
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Target: 5'- aGGCgUGGAGCGGauaccaaCAAGuuuacGCCGCa -3' miRNA: 3'- gUUGaACUUCGCCg------GUUCuuu--UGGCG- -5' |
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| 32038 | 5' | -48 | NC_006953.1 | + | 17782 | 0.69 | 0.822422 |
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Target: 5'- uUAGCgaugGAGGCGGCCGAGuuuuuAGCUucauuGCa -3' miRNA: 3'- -GUUGaa--CUUCGCCGGUUCuu---UUGG-----CG- -5' |
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| 32038 | 5' | -48 | NC_006953.1 | + | 24185 | 0.7 | 0.780752 |
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Target: 5'- --cCUUGAuGgGGCCAAGcuAACCGa -3' miRNA: 3'- guuGAACUuCgCCGGUUCuuUUGGCg -5' |
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| 32038 | 5' | -48 | NC_006953.1 | + | 35065 | 0.7 | 0.780752 |
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Target: 5'- uGGCUgGAGGCagaaaaGGCCAAGGAAACgGa -3' miRNA: 3'- gUUGAaCUUCG------CCGGUUCUUUUGgCg -5' |
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| 32038 | 5' | -48 | NC_006953.1 | + | 13254 | 0.71 | 0.73503 |
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Target: 5'- gAGCUUGAAGCGGCuuuagugCAGGGcaauauguaucuAAACuCGCu -3' miRNA: 3'- gUUGAACUUCGCCG-------GUUCU------------UUUG-GCG- -5' |
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| 32038 | 5' | -48 | NC_006953.1 | + | 20005 | 0.73 | 0.618095 |
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Target: 5'- gAACUUGcagaccuucaAAGCGGUCAAGuu-ACCGUa -3' miRNA: 3'- gUUGAAC----------UUCGCCGGUUCuuuUGGCG- -5' |
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| 32038 | 5' | -48 | NC_006953.1 | + | 17973 | 0.74 | 0.558751 |
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Target: 5'- gAACUUGAcGCaGCCAAcAAGGCCGUa -3' miRNA: 3'- gUUGAACUuCGcCGGUUcUUUUGGCG- -5' |
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| 32038 | 5' | -48 | NC_006953.1 | + | 18014 | 1.14 | 0.001641 |
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Target: 5'- gCAACUUGAAGCGGCCAAGAAAACCGCa -3' miRNA: 3'- -GUUGAACUUCGCCGGUUCUUUUGGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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