miRNA display CGI


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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
32047 3' -42.3 NC_006966.1 + 160538 0.68 1
Target:  5'- -------gGGUAUUAUACcuugaaaauggAACCCUCCa -3'
miRNA:   3'- cuaaaguaUCAUGAUAUG-----------UUGGGAGG- -5'
32047 3' -42.3 NC_006966.1 + 6539 0.69 1
Target:  5'- --gUUC-UGGUccauUACAACCCUCCu -3'
miRNA:   3'- cuaAAGuAUCAugauAUGUUGGGAGG- -5'
32047 3' -42.3 NC_006966.1 + 42969 0.66 1
Target:  5'- ---gUCaAUAGUAUa--ACAACCUUCCg -3'
miRNA:   3'- cuaaAG-UAUCAUGauaUGUUGGGAGG- -5'
32047 3' -42.3 NC_006966.1 + 125350 0.71 0.999984
Target:  5'- -----gAUAGUucuUUGUACAGCCUUCCu -3'
miRNA:   3'- cuaaagUAUCAu--GAUAUGUUGGGAGG- -5'
32047 3' -42.3 NC_006966.1 + 6767 1.16 0.030782
Target:  5'- gGAUUUCAUAGUACUAUACAACCCUCCa -3'
miRNA:   3'- -CUAAAGUAUCAUGAUAUGUUGGGAGG- -5'
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.