Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
32047 | 3' | -42.3 | NC_006966.1 | + | 160538 | 0.68 | 1 |
Target: 5'- -------gGGUAUUAUACcuugaaaauggAACCCUCCa -3' miRNA: 3'- cuaaaguaUCAUGAUAUG-----------UUGGGAGG- -5' |
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32047 | 3' | -42.3 | NC_006966.1 | + | 6539 | 0.69 | 1 |
Target: 5'- --gUUC-UGGUccauUACAACCCUCCu -3' miRNA: 3'- cuaAAGuAUCAugauAUGUUGGGAGG- -5' |
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32047 | 3' | -42.3 | NC_006966.1 | + | 42969 | 0.66 | 1 |
Target: 5'- ---gUCaAUAGUAUa--ACAACCUUCCg -3' miRNA: 3'- cuaaAG-UAUCAUGauaUGUUGGGAGG- -5' |
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32047 | 3' | -42.3 | NC_006966.1 | + | 125350 | 0.71 | 0.999984 |
Target: 5'- -----gAUAGUucuUUGUACAGCCUUCCu -3' miRNA: 3'- cuaaagUAUCAu--GAUAUGUUGGGAGG- -5' |
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32047 | 3' | -42.3 | NC_006966.1 | + | 6767 | 1.16 | 0.030782 |
Target: 5'- gGAUUUCAUAGUACUAUACAACCCUCCa -3' miRNA: 3'- -CUAAAGUAUCAUGAUAUGUUGGGAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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