Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
3207 | 3' | -52.7 | NC_001506.1 | + | 161 | 1.14 | 1.3e-05 |
Target: 5'- cGGACGAGCCCCCAAAUGAAAGACCCCc -3' miRNA: 3'- -CCUGCUCGGGGGUUUACUUUCUGGGG- -5' |
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3207 | 3' | -52.7 | NC_001506.1 | + | 2562 | 0.69 | 0.09648 |
Target: 5'- aGGAUGAgGCCaCCCuaggccaguGUGGcguaGAGGCCCUg -3' miRNA: 3'- -CCUGCU-CGG-GGGuu-------UACU----UUCUGGGG- -5' |
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3207 | 3' | -52.7 | NC_001506.1 | + | 3364 | 0.68 | 0.099947 |
Target: 5'- -uACGGGCUgUau--UGAAAGACCCCu -3' miRNA: 3'- ccUGCUCGGgGguuuACUUUCUGGGG- -5' |
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3207 | 3' | -52.7 | NC_001506.1 | + | 1500 | 0.66 | 0.155922 |
Target: 5'- gGGugGGGCgCCCAucguu---GCCCCg -3' miRNA: 3'- -CCugCUCGgGGGUuuacuuucUGGGG- -5' |
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3207 | 3' | -52.7 | NC_001506.1 | + | 909 | 0.66 | 0.156989 |
Target: 5'- aGGCaauAGCCuCCCAGGUGAcaauguauGGGACCUg -3' miRNA: 3'- cCUGc--UCGG-GGGUUUACU--------UUCUGGGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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