Results 1 - 11 of 11 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
32072 | 3' | -48.1 | NC_007016.1 | + | 15051 | 0.66 | 0.999247 |
Target: 5'- cCUGACGCCGGacgacuacgagaCGUUcgUGcUGAgcgGCGGa -3' miRNA: 3'- aGACUGUGGUC------------GUAAa-AC-ACUa--CGCC- -5' |
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32072 | 3' | -48.1 | NC_007016.1 | + | 106541 | 0.66 | 0.998313 |
Target: 5'- aUCUcGGCaACCAGCGg---GUGAUuCGGa -3' miRNA: 3'- -AGA-CUG-UGGUCGUaaaaCACUAcGCC- -5' |
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32072 | 3' | -48.1 | NC_007016.1 | + | 62589 | 0.66 | 0.998313 |
Target: 5'- -gUGGCGCaGGCGcgcuuggUUGUGcgGCGGg -3' miRNA: 3'- agACUGUGgUCGUaa-----AACACuaCGCC- -5' |
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32072 | 3' | -48.1 | NC_007016.1 | + | 119604 | 0.67 | 0.997605 |
Target: 5'- cCUGugGCCcaggcGGCGUUggGgaagcggaggccgcgGAUGCGGa -3' miRNA: 3'- aGACugUGG-----UCGUAAaaCa--------------CUACGCC- -5' |
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32072 | 3' | -48.1 | NC_007016.1 | + | 61357 | 0.67 | 0.997091 |
Target: 5'- -gUGAUACCcGCGg---GUGGUGgGGg -3' miRNA: 3'- agACUGUGGuCGUaaaaCACUACgCC- -5' |
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32072 | 3' | -48.1 | NC_007016.1 | + | 83592 | 0.69 | 0.988545 |
Target: 5'- uUCUG-UACUAGUg--UUGUGAUGgGGg -3' miRNA: 3'- -AGACuGUGGUCGuaaAACACUACgCC- -5' |
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32072 | 3' | -48.1 | NC_007016.1 | + | 116790 | 0.69 | 0.985174 |
Target: 5'- --cGGCACCGGCGUUaaccgGGUGCGu -3' miRNA: 3'- agaCUGUGGUCGUAAaaca-CUACGCc -5' |
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32072 | 3' | -48.1 | NC_007016.1 | + | 77001 | 0.7 | 0.983228 |
Target: 5'- --aGACGCCAGCGUguuuu--UGCGGg -3' miRNA: 3'- agaCUGUGGUCGUAaaacacuACGCC- -5' |
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32072 | 3' | -48.1 | NC_007016.1 | + | 96552 | 0.7 | 0.976228 |
Target: 5'- --cGACACUGGCAUg--GUGAUGUa- -3' miRNA: 3'- agaCUGUGGUCGUAaaaCACUACGcc -5' |
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32072 | 3' | -48.1 | NC_007016.1 | + | 45049 | 0.75 | 0.831117 |
Target: 5'- gUCcGGCACCGGCAUcggugcgGUGAUcGCGGu -3' miRNA: 3'- -AGaCUGUGGUCGUAaaa----CACUA-CGCC- -5' |
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32072 | 3' | -48.1 | NC_007016.1 | + | 10384 | 1.13 | 0.008162 |
Target: 5'- cUCUGACACCAGCAUUUUGUGAUGCGGa -3' miRNA: 3'- -AGACUGUGGUCGUAAAACACUACGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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