Results 1 - 20 of 27 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
32072 | 5' | -58.5 | NC_007016.1 | + | 114202 | 0.66 | 0.780893 |
Target: 5'- uGGCgGCUcCGGGugGCUccGGGUgGGCAc -3' miRNA: 3'- -CCGgCGGaGCUUugUGA--CCCGgUCGU- -5' |
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32072 | 5' | -58.5 | NC_007016.1 | + | 104415 | 0.66 | 0.780893 |
Target: 5'- cGGCaCGCC-C--AGCGcCUGGGCCAgGCGc -3' miRNA: 3'- -CCG-GCGGaGcuUUGU-GACCCGGU-CGU- -5' |
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32072 | 5' | -58.5 | NC_007016.1 | + | 124459 | 0.66 | 0.769725 |
Target: 5'- uGCCGCgUCGAugcccaucuuguACGCcuguucuccgUGGGCCAcGCAu -3' miRNA: 3'- cCGGCGgAGCUu-----------UGUG----------ACCCGGU-CGU- -5' |
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32072 | 5' | -58.5 | NC_007016.1 | + | 29089 | 0.66 | 0.751688 |
Target: 5'- uGGCUGCC-CGguAC-CUGGGUuuuuggaaccgggCAGCAg -3' miRNA: 3'- -CCGGCGGaGCuuUGuGACCCG-------------GUCGU- -5' |
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32072 | 5' | -58.5 | NC_007016.1 | + | 27210 | 0.67 | 0.733274 |
Target: 5'- aGCC-CCUCuuGAAACACUGauGGCCAagGCGa -3' miRNA: 3'- cCGGcGGAG--CUUUGUGAC--CCGGU--CGU- -5' |
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32072 | 5' | -58.5 | NC_007016.1 | + | 116987 | 0.67 | 0.723451 |
Target: 5'- aGGCCGUCUCcAGACGCgacGuGGCCAa-- -3' miRNA: 3'- -CCGGCGGAGcUUUGUGa--C-CCGGUcgu -5' |
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32072 | 5' | -58.5 | NC_007016.1 | + | 130322 | 0.67 | 0.714544 |
Target: 5'- cGCCGCCgggcucggccggacCGggGCGCUcccgggagucggcgGGGcCCGGCGg -3' miRNA: 3'- cCGGCGGa-------------GCuuUGUGA--------------CCC-GGUCGU- -5' |
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32072 | 5' | -58.5 | NC_007016.1 | + | 130508 | 0.67 | 0.713551 |
Target: 5'- gGGCCgGCCccgcCGAGGCGCcccGGGCCccAGCc -3' miRNA: 3'- -CCGG-CGGa---GCUUUGUGa--CCCGG--UCGu -5' |
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32072 | 5' | -58.5 | NC_007016.1 | + | 69213 | 0.67 | 0.707577 |
Target: 5'- cGGCUGCaCUaauccacgcagcgucCGAAGCGuCUGuGGCCgAGCAa -3' miRNA: 3'- -CCGGCG-GA---------------GCUUUGU-GAC-CCGG-UCGU- -5' |
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32072 | 5' | -58.5 | NC_007016.1 | + | 51138 | 0.67 | 0.703582 |
Target: 5'- aGGCCGCUgCGuuAAGCAUuuUGGcaGCCGGCGu -3' miRNA: 3'- -CCGGCGGaGC--UUUGUG--ACC--CGGUCGU- -5' |
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32072 | 5' | -58.5 | NC_007016.1 | + | 16717 | 0.67 | 0.683472 |
Target: 5'- cGCCGCCggaaucccgUGggGCGaugaGGGCCAcGCAg -3' miRNA: 3'- cCGGCGGa--------GCuuUGUga--CCCGGU-CGU- -5' |
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32072 | 5' | -58.5 | NC_007016.1 | + | 20542 | 0.67 | 0.683472 |
Target: 5'- gGGaCCGCCgCGAgggcGAC-CUGGGgCCGGUg -3' miRNA: 3'- -CC-GGCGGaGCU----UUGuGACCC-GGUCGu -5' |
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32072 | 5' | -58.5 | NC_007016.1 | + | 20709 | 0.68 | 0.67335 |
Target: 5'- cGGCCGUC-CGggGCACgc-GCCgcGGCAa -3' miRNA: 3'- -CCGGCGGaGCuuUGUGaccCGG--UCGU- -5' |
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32072 | 5' | -58.5 | NC_007016.1 | + | 31150 | 0.68 | 0.663194 |
Target: 5'- uGCuCGCuCUCGGAAgaaaACUGGGCCccGGUAa -3' miRNA: 3'- cCG-GCG-GAGCUUUg---UGACCCGG--UCGU- -5' |
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32072 | 5' | -58.5 | NC_007016.1 | + | 21840 | 0.68 | 0.663194 |
Target: 5'- gGGuCCGCCUCGAcggaAACcagGC-GuGGCCAGCc -3' miRNA: 3'- -CC-GGCGGAGCU----UUG---UGaC-CCGGUCGu -5' |
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32072 | 5' | -58.5 | NC_007016.1 | + | 13328 | 0.68 | 0.63261 |
Target: 5'- cGCCuGCCUCcGAACGCcggGGGCaGGCGg -3' miRNA: 3'- cCGG-CGGAGcUUUGUGa--CCCGgUCGU- -5' |
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32072 | 5' | -58.5 | NC_007016.1 | + | 125245 | 0.68 | 0.621383 |
Target: 5'- aGGCCGCCccgCGAGAC-CaGGGCUcccagguGGCu -3' miRNA: 3'- -CCGGCGGa--GCUUUGuGaCCCGG-------UCGu -5' |
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32072 | 5' | -58.5 | NC_007016.1 | + | 126473 | 0.69 | 0.612205 |
Target: 5'- aGCCuCCUCGucuGCACUaaGGGCCgcGGCGg -3' miRNA: 3'- cCGGcGGAGCuu-UGUGA--CCCGG--UCGU- -5' |
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32072 | 5' | -58.5 | NC_007016.1 | + | 68338 | 0.69 | 0.612205 |
Target: 5'- cGGCCGCCaugCGGGACuCUGGaguGUUGGCGa -3' miRNA: 3'- -CCGGCGGa--GCUUUGuGACC---CGGUCGU- -5' |
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32072 | 5' | -58.5 | NC_007016.1 | + | 40976 | 0.69 | 0.602021 |
Target: 5'- gGGCCGggggaaCguuaaacgCGAccggcacGCGCUGGGCCGGCAg -3' miRNA: 3'- -CCGGCg-----Ga-------GCUu------UGUGACCCGGUCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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