Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32074 | 3' | -59 | NC_007016.1 | + | 59008 | 0.71 | 0.490254 |
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Target: 5'- aCGCGUCUccuuUGAUAG-CCCCCUGUaCCg -3' miRNA: 3'- aGCGCGGG----ACUAUUgGGGGGACAgGG- -5' |
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| 32074 | 3' | -59 | NC_007016.1 | + | 130492 | 0.72 | 0.426679 |
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Target: 5'- -gGCGCCCcGGgccccAGCCCCCC--UCCCg -3' miRNA: 3'- agCGCGGGaCUa----UUGGGGGGacAGGG- -5' |
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| 32074 | 3' | -59 | NC_007016.1 | + | 51284 | 0.72 | 0.409427 |
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Target: 5'- gUCGCGCCCUGAUAuUCCCgCaGUUaaCCg -3' miRNA: 3'- -AGCGCGGGACUAUuGGGGgGaCAG--GG- -5' |
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| 32074 | 3' | -59 | NC_007016.1 | + | 68000 | 0.73 | 0.352606 |
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Target: 5'- cCGCaGCCC-GAUAuACCgCCCCaUGUCCCc -3' miRNA: 3'- aGCG-CGGGaCUAU-UGG-GGGG-ACAGGG- -5' |
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| 32074 | 3' | -59 | NC_007016.1 | + | 130193 | 0.77 | 0.227739 |
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Target: 5'- cCGCGCCCcGAc-GCCCCCCgagCCCg -3' miRNA: 3'- aGCGCGGGaCUauUGGGGGGacaGGG- -5' |
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| 32074 | 3' | -59 | NC_007016.1 | + | 15040 | 1.11 | 0.000943 |
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Target: 5'- gUCGCGCCCUGAUAACCCCCCUGUCCCu -3' miRNA: 3'- -AGCGCGGGACUAUUGGGGGGACAGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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