Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value![]() |
Predicted miRNA align pattern | |||||||
32076 | 5' | -51 | NC_007016.1 | + | 97391 | 0.68 | 0.960291 |
Target: 5'- aUCUGUCGcGCuuaUCugGAG-ACGGCUg -3' miRNA: 3'- aAGAUAGC-CGca-AGugCUCuUGUCGA- -5' |
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32076 | 5' | -51 | NC_007016.1 | + | 125645 | 0.69 | 0.936717 |
Target: 5'- cUCUGU-GGCGUgCGCGAGAaaaugcgcccacugACGGCg -3' miRNA: 3'- aAGAUAgCCGCAaGUGCUCU--------------UGUCGa -5' |
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32076 | 5' | -51 | NC_007016.1 | + | 110958 | 0.69 | 0.915864 |
Target: 5'- gUCaGUUGGUGUgCACGAcGAACAGUUg -3' miRNA: 3'- aAGaUAGCCGCAaGUGCU-CUUGUCGA- -5' |
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32076 | 5' | -51 | NC_007016.1 | + | 101896 | 0.7 | 0.896284 |
Target: 5'- ---cGUUGGCGUguagucCAUGGGAACGGCg -3' miRNA: 3'- aagaUAGCCGCAa-----GUGCUCUUGUCGa -5' |
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32076 | 5' | -51 | NC_007016.1 | + | 70043 | 0.72 | 0.824249 |
Target: 5'- gUCUGggUGGCGUggauugCACGGGGACGGUc -3' miRNA: 3'- aAGAUa-GCCGCAa-----GUGCUCUUGUCGa -5' |
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32076 | 5' | -51 | NC_007016.1 | + | 16385 | 1.05 | 0.011264 |
Target: 5'- uUUCUAUCGGCGUUCACGAGAACAGCUu -3' miRNA: 3'- -AAGAUAGCCGCAAGUGCUCUUGUCGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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