Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32079 | 5' | -55.7 | NC_007016.1 | + | 65323 | 0.66 | 0.87354 |
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Target: 5'- cGGCGCucguGCAGCUCUGACagccgcaCGCCAg -3' miRNA: 3'- -CUGCG----UGUCGAGGCUGgacaa--GUGGU- -5' |
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| 32079 | 5' | -55.7 | NC_007016.1 | + | 99056 | 0.66 | 0.866023 |
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Target: 5'- ---uUACGGCUCCGGCUUugUCGCCGc -3' miRNA: 3'- cugcGUGUCGAGGCUGGAcaAGUGGU- -5' |
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| 32079 | 5' | -55.7 | NC_007016.1 | + | 16133 | 0.68 | 0.770541 |
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Target: 5'- --gGgGCAGCUCCucgcACCUGcUCGCCAg -3' miRNA: 3'- cugCgUGUCGAGGc---UGGACaAGUGGU- -5' |
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| 32079 | 5' | -55.7 | NC_007016.1 | + | 130234 | 0.69 | 0.751166 |
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Target: 5'- cGGCGCGCGGCUCCcgauGCCgggcGgcCGCCGc -3' miRNA: 3'- -CUGCGUGUCGAGGc---UGGa---CaaGUGGU- -5' |
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| 32079 | 5' | -55.7 | NC_007016.1 | + | 21502 | 0.69 | 0.741311 |
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Target: 5'- uGGCGCACAGCuUCUGACCgcgGUgggUCuCUAa -3' miRNA: 3'- -CUGCGUGUCG-AGGCUGGa--CA---AGuGGU- -5' |
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| 32079 | 5' | -55.7 | NC_007016.1 | + | 17870 | 1.08 | 0.002658 |
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Target: 5'- uGACGCACAGCUCCGACCUGUUCACCAg -3' miRNA: 3'- -CUGCGUGUCGAGGCUGGACAAGUGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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