Results 1 - 20 of 22 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
32081 | 5' | -57.3 | NC_007016.1 | + | 41318 | 0.66 | 0.807226 |
Target: 5'- -cGGAUGCCaacgauucgGUgGUCCG-GGCCAGauucGCg -3' miRNA: 3'- uaCCUGCGG---------CAgUAGGCaCCGGUU----CG- -5' |
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32081 | 5' | -57.3 | NC_007016.1 | + | 41559 | 0.66 | 0.807226 |
Target: 5'- --cGugGCCaGcUGUCCGUGGCUGAGUc -3' miRNA: 3'- uacCugCGG-CaGUAGGCACCGGUUCG- -5' |
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32081 | 5' | -57.3 | NC_007016.1 | + | 124478 | 0.66 | 0.807226 |
Target: 5'- uUGuACGCCuGUUcUCCGUGGgCCAcGCa -3' miRNA: 3'- uACcUGCGG-CAGuAGGCACC-GGUuCG- -5' |
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32081 | 5' | -57.3 | NC_007016.1 | + | 51878 | 0.66 | 0.798267 |
Target: 5'- -gGGACGacaUGUCAaUCGUgaGGCCGAGUu -3' miRNA: 3'- uaCCUGCg--GCAGUaGGCA--CCGGUUCG- -5' |
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32081 | 5' | -57.3 | NC_007016.1 | + | 11752 | 0.67 | 0.789156 |
Target: 5'- -aGGuuGCCGUCAcgUCGuUGGCCAcgaAGUg -3' miRNA: 3'- uaCCugCGGCAGUa-GGC-ACCGGU---UCG- -5' |
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32081 | 5' | -57.3 | NC_007016.1 | + | 80882 | 0.67 | 0.779902 |
Target: 5'- uGUGGagGCGCCGUCccGUCCcgagguguucUGGCCAuGCc -3' miRNA: 3'- -UACC--UGCGGCAG--UAGGc---------ACCGGUuCG- -5' |
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32081 | 5' | -57.3 | NC_007016.1 | + | 122374 | 0.67 | 0.770515 |
Target: 5'- -gGGACGUaaUCAg-CGUGGCCAuGCa -3' miRNA: 3'- uaCCUGCGgcAGUagGCACCGGUuCG- -5' |
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32081 | 5' | -57.3 | NC_007016.1 | + | 116133 | 0.67 | 0.74164 |
Target: 5'- -cGGugGCCGggCAgaggCCGgccGGCCAaaGGUg -3' miRNA: 3'- uaCCugCGGCa-GUa---GGCa--CCGGU--UCG- -5' |
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32081 | 5' | -57.3 | NC_007016.1 | + | 123613 | 0.67 | 0.738701 |
Target: 5'- -cGG-UGCCGUCGaggccaccggCCGUGGCCGcccugaucgcccccGGCg -3' miRNA: 3'- uaCCuGCGGCAGUa---------GGCACCGGU--------------UCG- -5' |
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32081 | 5' | -57.3 | NC_007016.1 | + | 78453 | 0.68 | 0.70183 |
Target: 5'- -cGGACGCC----UCgGUGGCCGGGg -3' miRNA: 3'- uaCCUGCGGcaguAGgCACCGGUUCg -5' |
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32081 | 5' | -57.3 | NC_007016.1 | + | 115701 | 0.69 | 0.671316 |
Target: 5'- uUGG-CGCCGgggUCAUCCGgcGGUCAGcGCa -3' miRNA: 3'- uACCuGCGGC---AGUAGGCa-CCGGUU-CG- -5' |
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32081 | 5' | -57.3 | NC_007016.1 | + | 77605 | 0.69 | 0.661068 |
Target: 5'- -cGGAagUGCCacaUCGaaCCGUGGCCGGGCg -3' miRNA: 3'- uaCCU--GCGGc--AGUa-GGCACCGGUUCG- -5' |
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32081 | 5' | -57.3 | NC_007016.1 | + | 12663 | 0.69 | 0.64051 |
Target: 5'- uUGGcCGCacgGUCGUUaaCGUGGCCAguAGCg -3' miRNA: 3'- uACCuGCGg--CAGUAG--GCACCGGU--UCG- -5' |
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32081 | 5' | -57.3 | NC_007016.1 | + | 13907 | 0.7 | 0.599379 |
Target: 5'- cGUGGACGCCGUUcgCCGguucguccUGGaucaCGGGUu -3' miRNA: 3'- -UACCUGCGGCAGuaGGC--------ACCg---GUUCG- -5' |
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32081 | 5' | -57.3 | NC_007016.1 | + | 37752 | 0.7 | 0.589138 |
Target: 5'- -cGaGACGUuuucaagaaucuCGUUAUUgGUGGCCAGGCa -3' miRNA: 3'- uaC-CUGCG------------GCAGUAGgCACCGGUUCG- -5' |
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32081 | 5' | -57.3 | NC_007016.1 | + | 101307 | 0.7 | 0.578929 |
Target: 5'- -aGGAUGCCGUCgAUCU-UGGCCAGa- -3' miRNA: 3'- uaCCUGCGGCAG-UAGGcACCGGUUcg -5' |
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32081 | 5' | -57.3 | NC_007016.1 | + | 101861 | 0.71 | 0.508894 |
Target: 5'- -cGGACgGCCGUUGcUCGUGGCCGucgaauugGGCg -3' miRNA: 3'- uaCCUG-CGGCAGUaGGCACCGGU--------UCG- -5' |
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32081 | 5' | -57.3 | NC_007016.1 | + | 17887 | 0.72 | 0.470487 |
Target: 5'- cUGGACaUCGUacCcgCCGUGGUCGAGCg -3' miRNA: 3'- uACCUGcGGCA--GuaGGCACCGGUUCG- -5' |
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32081 | 5' | -57.3 | NC_007016.1 | + | 40146 | 0.72 | 0.461116 |
Target: 5'- -cGGACGCUGgccugCGUCCcaccaccucGUGGCCA-GCa -3' miRNA: 3'- uaCCUGCGGCa----GUAGG---------CACCGGUuCG- -5' |
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32081 | 5' | -57.3 | NC_007016.1 | + | 13593 | 0.73 | 0.424656 |
Target: 5'- uUGGccgcGCGCCGUCcacauggaccGUUCGUGGgCGAGCa -3' miRNA: 3'- uACC----UGCGGCAG----------UAGGCACCgGUUCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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