Results 21 - 30 of 30 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32086 | 5' | -59.5 | NC_007016.1 | + | 19213 | 0.69 | 0.581338 |
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Target: 5'- aUGGCCGcggcgCCCG-CGUuuccggGCGCGUCGUCc -3' miRNA: 3'- gACUGGCa----GGGCaGCA------UGCGCGGCGG- -5' |
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| 32086 | 5' | -59.5 | NC_007016.1 | + | 67974 | 0.7 | 0.52321 |
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Target: 5'- uUGACUGcgggaaaCCCGUa-UGCGCGCCGCa -3' miRNA: 3'- gACUGGCa------GGGCAgcAUGCGCGGCGg -5' |
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| 32086 | 5' | -59.5 | NC_007016.1 | + | 84450 | 0.71 | 0.504312 |
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Target: 5'- cCUGugUGUCCgCG-CGUggACGCGCuuCGCCu -3' miRNA: 3'- -GACugGCAGG-GCaGCA--UGCGCG--GCGG- -5' |
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| 32086 | 5' | -59.5 | NC_007016.1 | + | 13603 | 0.71 | 0.476539 |
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Target: 5'- -cGACUGg-CCGUUGgccGCGCGCCGUCc -3' miRNA: 3'- gaCUGGCagGGCAGCa--UGCGCGGCGG- -5' |
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| 32086 | 5' | -59.5 | NC_007016.1 | + | 14939 | 0.71 | 0.476539 |
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Target: 5'- uUGGCCGU-CCGUUaggACGCGCCugGCCu -3' miRNA: 3'- gACUGGCAgGGCAGca-UGCGCGG--CGG- -5' |
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| 32086 | 5' | -59.5 | NC_007016.1 | + | 130393 | 0.73 | 0.398222 |
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Target: 5'- -cGGCCG-CCCGgcaUCGggagccGCGCGCCGCg -3' miRNA: 3'- gaCUGGCaGGGC---AGCa-----UGCGCGGCGg -5' |
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| 32086 | 5' | -59.5 | NC_007016.1 | + | 130051 | 0.73 | 0.390039 |
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Target: 5'- -cGGCCGcCCCG-CGccGCGCGCCggGCCg -3' miRNA: 3'- gaCUGGCaGGGCaGCa-UGCGCGG--CGG- -5' |
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| 32086 | 5' | -59.5 | NC_007016.1 | + | 20709 | 0.73 | 0.358429 |
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Target: 5'- -cGGCCGUCCgGg-GcACGCGCCGCg -3' miRNA: 3'- gaCUGGCAGGgCagCaUGCGCGGCGg -5' |
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| 32086 | 5' | -59.5 | NC_007016.1 | + | 17995 | 0.75 | 0.307551 |
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Target: 5'- -cGAUCGUCaCUGUgGUGCGC-CCGCCc -3' miRNA: 3'- gaCUGGCAG-GGCAgCAUGCGcGGCGG- -5' |
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| 32086 | 5' | -59.5 | NC_007016.1 | + | 26874 | 1.11 | 0.001148 |
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Target: 5'- aCUGACCGUCCCGUCGUACGCGCCGCCc -3' miRNA: 3'- -GACUGGCAGGGCAGCAUGCGCGGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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