Results 1 - 20 of 22 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
32087 | 5' | -55.9 | NC_007016.1 | + | 57633 | 0.66 | 0.857931 |
Target: 5'- cUCGuGCUCgaccacacACGCAAACACaaACCCGGu -3' miRNA: 3'- aAGC-CGAG--------UGCGUUUGUGgcUGGGUU- -5' |
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32087 | 5' | -55.9 | NC_007016.1 | + | 8970 | 0.66 | 0.857931 |
Target: 5'- cUCGGCUCGaauaucuuugcCGCAAACugCGcucuagcucCCCGAg -3' miRNA: 3'- aAGCCGAGU-----------GCGUUUGugGCu--------GGGUU- -5' |
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32087 | 5' | -55.9 | NC_007016.1 | + | 130523 | 0.66 | 0.857931 |
Target: 5'- cUCGG-UCGCGCcccGGgGCCGGCCCc- -3' miRNA: 3'- aAGCCgAGUGCGu--UUgUGGCUGGGuu -5' |
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32087 | 5' | -55.9 | NC_007016.1 | + | 116131 | 0.66 | 0.857931 |
Target: 5'- cUCGGUggcCGgGCAGAgGCCGGCCg-- -3' miRNA: 3'- aAGCCGa--GUgCGUUUgUGGCUGGguu -5' |
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32087 | 5' | -55.9 | NC_007016.1 | + | 15438 | 0.66 | 0.857931 |
Target: 5'- -gCGGUUUcgcgucgucuACGguGACACCGACUCGc -3' miRNA: 3'- aaGCCGAG----------UGCguUUGUGGCUGGGUu -5' |
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32087 | 5' | -55.9 | NC_007016.1 | + | 17136 | 0.66 | 0.84987 |
Target: 5'- -gCGGC-CGCGUAuuggggguguACGCCGACgCCGAa -3' miRNA: 3'- aaGCCGaGUGCGUu---------UGUGGCUG-GGUU- -5' |
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32087 | 5' | -55.9 | NC_007016.1 | + | 71696 | 0.66 | 0.833124 |
Target: 5'- --gGGCUaACGCGAGCACaauuGACCCc- -3' miRNA: 3'- aagCCGAgUGCGUUUGUGg---CUGGGuu -5' |
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32087 | 5' | -55.9 | NC_007016.1 | + | 85643 | 0.66 | 0.833124 |
Target: 5'- --gGGCUCucccGCGCAGGCugC-ACCCGGa -3' miRNA: 3'- aagCCGAG----UGCGUUUGugGcUGGGUU- -5' |
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32087 | 5' | -55.9 | NC_007016.1 | + | 117479 | 0.67 | 0.815602 |
Target: 5'- gUUGGCaaGCGCc-GCACCGgACCCAu -3' miRNA: 3'- aAGCCGagUGCGuuUGUGGC-UGGGUu -5' |
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32087 | 5' | -55.9 | NC_007016.1 | + | 116507 | 0.67 | 0.797371 |
Target: 5'- aUCGGCgccaccucacUCACGCAAGCggcguACCGGCgCGc -3' miRNA: 3'- aAGCCG----------AGUGCGUUUG-----UGGCUGgGUu -5' |
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32087 | 5' | -55.9 | NC_007016.1 | + | 40566 | 0.67 | 0.777546 |
Target: 5'- --gGGUUCAcgacgucCGCAAACACCGGgCCGu -3' miRNA: 3'- aagCCGAGU-------GCGUUUGUGGCUgGGUu -5' |
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32087 | 5' | -55.9 | NC_007016.1 | + | 118187 | 0.67 | 0.768858 |
Target: 5'- -gCGGCUCACGgAGGCuuaauaccaacgACCGGCUUAAc -3' miRNA: 3'- aaGCCGAGUGCgUUUG------------UGGCUGGGUU- -5' |
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32087 | 5' | -55.9 | NC_007016.1 | + | 27536 | 0.68 | 0.759081 |
Target: 5'- -aCGGCUCGCGgGGgacucGCGagCGGCCCAAa -3' miRNA: 3'- aaGCCGAGUGCgUU-----UGUg-GCUGGGUU- -5' |
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32087 | 5' | -55.9 | NC_007016.1 | + | 42308 | 0.68 | 0.73918 |
Target: 5'- -gCGGCUgACGC--GCAUCGAuCCCAu -3' miRNA: 3'- aaGCCGAgUGCGuuUGUGGCU-GGGUu -5' |
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32087 | 5' | -55.9 | NC_007016.1 | + | 124495 | 0.68 | 0.718881 |
Target: 5'- gUgGGC-CACGCAuuguCCGACCCAc -3' miRNA: 3'- aAgCCGaGUGCGUuuguGGCUGGGUu -5' |
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32087 | 5' | -55.9 | NC_007016.1 | + | 92541 | 0.69 | 0.712727 |
Target: 5'- -cUGGCUCugGUcuacaaacggagcccGcGCACCGGCCCGc -3' miRNA: 3'- aaGCCGAGugCG---------------UuUGUGGCUGGGUu -5' |
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32087 | 5' | -55.9 | NC_007016.1 | + | 81314 | 0.69 | 0.708609 |
Target: 5'- -cCGGCUCccacCGCAccaguguacugAACACCGGCaCCAAc -3' miRNA: 3'- aaGCCGAGu---GCGU-----------UUGUGGCUG-GGUU- -5' |
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32087 | 5' | -55.9 | NC_007016.1 | + | 93243 | 0.69 | 0.656408 |
Target: 5'- cUCGGCUUugGCuaccuuGACGCCGagaGCgCCAAg -3' miRNA: 3'- aAGCCGAGugCGu-----UUGUGGC---UG-GGUU- -5' |
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32087 | 5' | -55.9 | NC_007016.1 | + | 40884 | 0.7 | 0.635317 |
Target: 5'- aUUGGCcUACGCuAACACCuGCCCGAu -3' miRNA: 3'- aAGCCGaGUGCGuUUGUGGcUGGGUU- -5' |
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32087 | 5' | -55.9 | NC_007016.1 | + | 43489 | 0.7 | 0.624763 |
Target: 5'- aUUGGgUUAacCGCAAACACCaGACCCGGu -3' miRNA: 3'- aAGCCgAGU--GCGUUUGUGG-CUGGGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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