Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32088 | 3' | -61.9 | NC_007016.1 | + | 110888 | 0.66 | 0.630759 |
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Target: 5'- cACC-GACCCGGGGCga-UGGUuuuuagguuuaaCGGCa -3' miRNA: 3'- -UGGaCUGGGUCCCGagcACCA------------GCCGc -5' |
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| 32088 | 3' | -61.9 | NC_007016.1 | + | 126494 | 0.66 | 0.61094 |
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Target: 5'- gGCCgcggcGGCCCagcgAGGGUUCGUGcGUUGGg- -3' miRNA: 3'- -UGGa----CUGGG----UCCCGAGCAC-CAGCCgc -5' |
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| 32088 | 3' | -61.9 | NC_007016.1 | + | 115598 | 0.66 | 0.591179 |
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Target: 5'- aGCCgGGCgCGGGGCUCGacGGcugUGGCa -3' miRNA: 3'- -UGGaCUGgGUCCCGAGCa-CCa--GCCGc -5' |
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| 32088 | 3' | -61.9 | NC_007016.1 | + | 124682 | 0.67 | 0.57545 |
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Target: 5'- aACCUGggucACCCAGGGCagGggcagacucaggugGGUCGGaCa -3' miRNA: 3'- -UGGAC----UGGGUCCCGagCa-------------CCAGCC-Gc -5' |
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| 32088 | 3' | -61.9 | NC_007016.1 | + | 130362 | 0.67 | 0.571531 |
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Target: 5'- gGCUUGugggGCCCcGGGCUCGgggggcgucgGGgcgCGGCGc -3' miRNA: 3'- -UGGAC----UGGGuCCCGAGCa---------CCa--GCCGC- -5' |
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| 32088 | 3' | -61.9 | NC_007016.1 | + | 130344 | 0.69 | 0.449547 |
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Target: 5'- gGCUggGGCCCGGGGCgccUCGgcggGGcCGGCc -3' miRNA: 3'- -UGGa-CUGGGUCCCG---AGCa---CCaGCCGc -5' |
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| 32088 | 3' | -61.9 | NC_007016.1 | + | 130309 | 0.7 | 0.406514 |
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Target: 5'- gGCCgGACCgGGGcGCUCccggGaGUCGGCGg -3' miRNA: 3'- -UGGaCUGGgUCC-CGAGca--C-CAGCCGC- -5' |
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| 32088 | 3' | -61.9 | NC_007016.1 | + | 28315 | 1.07 | 0.001003 |
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Target: 5'- aACCUGACCCAGGGCUCGUGGUCGGCGu -3' miRNA: 3'- -UGGACUGGGUCCCGAGCACCAGCCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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