Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32089 | 5' | -58.3 | NC_007016.1 | + | 21247 | 0.66 | 0.772714 |
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Target: 5'- cUCCGGGGgagacgcuggGGGAGACuCCGggggagacgcCGGGGg -3' miRNA: 3'- aAGGUUCCa---------CCUUCUG-GGCa---------GCCCCa -5' |
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| 32089 | 5' | -58.3 | NC_007016.1 | + | 21199 | 0.66 | 0.772714 |
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Target: 5'- cUCCGGGGgagacgccggGGGAGACuCCGggggagacgcCGGGGg -3' miRNA: 3'- aAGGUUCCa---------CCUUCUG-GGCa---------GCCCCa -5' |
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| 32089 | 5' | -58.3 | NC_007016.1 | + | 130085 | 0.67 | 0.704528 |
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Target: 5'- gUUCgCGGGGccGGggGuCCCG-CGGGGg -3' miRNA: 3'- -AAG-GUUCCa-CCuuCuGGGCaGCCCCa -5' |
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| 32089 | 5' | -58.3 | NC_007016.1 | + | 89010 | 0.69 | 0.611825 |
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Target: 5'- -gUUAAGGUGGguGACCCGaaacgcuUUGGGGg -3' miRNA: 3'- aaGGUUCCACCuuCUGGGC-------AGCCCCa -5' |
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| 32089 | 5' | -58.3 | NC_007016.1 | + | 123080 | 0.7 | 0.512426 |
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Target: 5'- cUCC-AGGUGGAuGuCCCacugGUCGGGGg -3' miRNA: 3'- aAGGuUCCACCUuCuGGG----CAGCCCCa -5' |
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| 32089 | 5' | -58.3 | NC_007016.1 | + | 29014 | 1.04 | 0.002975 |
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Target: 5'- uUUCCAAGGUGGAAGACCCGUCGGGGUc -3' miRNA: 3'- -AAGGUUCCACCUUCUGGGCAGCCCCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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