Results 1 - 7 of 7 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
32091 | 3' | -53.6 | NC_007016.1 | + | 123016 | 0.66 | 0.956299 |
Target: 5'- uGAugGUGGUCugGGGGAUgUGgaGGUUUa -3' miRNA: 3'- uUUugUACCAG--CCCCUAgACg-CCAGG- -5' |
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32091 | 3' | -53.6 | NC_007016.1 | + | 119647 | 0.66 | 0.933655 |
Target: 5'- ------gGGUCGGGG-UCUGa-GUCCg -3' miRNA: 3'- uuuuguaCCAGCCCCuAGACgcCAGG- -5' |
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32091 | 3' | -53.6 | NC_007016.1 | + | 20310 | 0.67 | 0.928915 |
Target: 5'- --uACGUGGcUCGGGGGgagcugucaugccagCUGUaccagaGGUCCg -3' miRNA: 3'- uuuUGUACC-AGCCCCUa--------------GACG------CCAGG- -5' |
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32091 | 3' | -53.6 | NC_007016.1 | + | 130313 | 0.67 | 0.907278 |
Target: 5'- cGAAAgGUGGUCuccgcgccgccgggaGGGGggCUGgGGcCCg -3' miRNA: 3'- -UUUUgUACCAG---------------CCCCuaGACgCCaGG- -5' |
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32091 | 3' | -53.6 | NC_007016.1 | + | 130088 | 0.69 | 0.861932 |
Target: 5'- --cGCggGGcCGGGGGUCccGCGGggggCCg -3' miRNA: 3'- uuuUGuaCCaGCCCCUAGa-CGCCa---GG- -5' |
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32091 | 3' | -53.6 | NC_007016.1 | + | 14218 | 0.73 | 0.641188 |
Target: 5'- -cGAUgcGGUaCGGGGGccagucgCUGCGGUCCg -3' miRNA: 3'- uuUUGuaCCA-GCCCCUa------GACGCCAGG- -5' |
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32091 | 3' | -53.6 | NC_007016.1 | + | 29923 | 1.09 | 0.004076 |
Target: 5'- cAAAACAUGGUCGGGGAUCUGCGGUCCc -3' miRNA: 3'- -UUUUGUACCAGCCCCUAGACGCCAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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