Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
32094 | 5' | -53.6 | NC_007016.1 | + | 52637 | 0.66 | 0.938275 |
Target: 5'- gGCCgCGCGUUACCUGUucuaucaaUGCAcacacGUGGUg -3' miRNA: 3'- -CGG-GUGCGAUGGACA--------GCGUu----CAUCAa -5' |
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32094 | 5' | -53.6 | NC_007016.1 | + | 40772 | 0.67 | 0.903835 |
Target: 5'- gGCUCACGUaucucCCgucGUCGUGAGUGGUUa -3' miRNA: 3'- -CGGGUGCGau---GGa--CAGCGUUCAUCAA- -5' |
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32094 | 5' | -53.6 | NC_007016.1 | + | 13093 | 0.72 | 0.647449 |
Target: 5'- cGCCUACGCUcuuCCUGUUGUAGcUGGUg -3' miRNA: 3'- -CGGGUGCGAu--GGACAGCGUUcAUCAa -5' |
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32094 | 5' | -53.6 | NC_007016.1 | + | 31981 | 1.07 | 0.004545 |
Target: 5'- cGCCCACGCUACCUGUCGCAAGUAGUUu -3' miRNA: 3'- -CGGGUGCGAUGGACAGCGUUCAUCAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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