Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32098 | 5' | -56.8 | NC_007016.1 | + | 130471 | 0.66 | 0.86885 |
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Target: 5'- ------cCUCCCGGC-GGCGCGgagACc -3' miRNA: 3'- cuacaaaGAGGGCCGcCCGCGCa--UGc -5' |
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| 32098 | 5' | -56.8 | NC_007016.1 | + | 26705 | 0.66 | 0.86885 |
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Target: 5'- cGGUGgcgCUaCgGGC-GGCGCGUACGa -3' miRNA: 3'- -CUACaaaGAgGgCCGcCCGCGCAUGC- -5' |
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| 32098 | 5' | -56.8 | NC_007016.1 | + | 19173 | 0.66 | 0.853617 |
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Target: 5'- aGAUGUUUUUgCgGGaCGGGCGCc-GCGc -3' miRNA: 3'- -CUACAAAGAgGgCC-GCCCGCGcaUGC- -5' |
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| 32098 | 5' | -56.8 | NC_007016.1 | + | 22548 | 0.67 | 0.80334 |
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Target: 5'- --aGUUUCcacggucgCCCGGCGcGCGCGUGg- -3' miRNA: 3'- cuaCAAAGa-------GGGCCGCcCGCGCAUgc -5' |
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| 32098 | 5' | -56.8 | NC_007016.1 | + | 20886 | 0.67 | 0.794372 |
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Target: 5'- --cGUUUCUCgCGGauccgagguugcCGcGGCGCGUGCc -3' miRNA: 3'- cuaCAAAGAGgGCC------------GC-CCGCGCAUGc -5' |
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| 32098 | 5' | -56.8 | NC_007016.1 | + | 111017 | 0.68 | 0.766624 |
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Target: 5'- -cUGUUUUUUCUGGCGGaaucuucgccgaGCGCGUuuGCGu -3' miRNA: 3'- cuACAAAGAGGGCCGCC------------CGCGCA--UGC- -5' |
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| 32098 | 5' | -56.8 | NC_007016.1 | + | 26305 | 0.69 | 0.727998 |
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Target: 5'- -----cUCUgcccCCCGcGCGGGUGCGUGCc -3' miRNA: 3'- cuacaaAGA----GGGC-CGCCCGCGCAUGc -5' |
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| 32098 | 5' | -56.8 | NC_007016.1 | + | 41929 | 0.69 | 0.698127 |
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Target: 5'- uGGUGgcaggcgUUCCCgugaGGCGGGCcGCGUACu -3' miRNA: 3'- -CUACaaa----GAGGG----CCGCCCG-CGCAUGc -5' |
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| 32098 | 5' | -56.8 | NC_007016.1 | + | 118849 | 0.7 | 0.647354 |
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Target: 5'- aAUGggguaUCgCGGCGGGCGCGggggGCGc -3' miRNA: 3'- cUACaaag-AGgGCCGCCCGCGCa---UGC- -5' |
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| 32098 | 5' | -56.8 | NC_007016.1 | + | 51361 | 1.09 | 0.002296 |
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Target: 5'- gGAUGUUUCUCCCGGCGGGCGCGUACGa -3' miRNA: 3'- -CUACAAAGAGGGCCGCCCGCGCAUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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