Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32099 | 3' | -47.6 | NC_007016.1 | + | 35791 | 0.66 | 0.999239 |
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Target: 5'- cGgUCGUUgcuaUGAAAGAcauaaaauUGGCGUUGCg -3' miRNA: 3'- -CgAGUAAaa--GCUUUCU--------ACCGCGACGa -5' |
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| 32099 | 3' | -47.6 | NC_007016.1 | + | 91648 | 0.66 | 0.998843 |
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Target: 5'- aCUCGUc-UCGGGAGAucaUGGUGUUGUa -3' miRNA: 3'- cGAGUAaaAGCUUUCU---ACCGCGACGa -5' |
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| 32099 | 3' | -47.6 | NC_007016.1 | + | 17901 | 0.66 | 0.99882 |
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Target: 5'- cGC-CGUggUCGAgcgcgcggaccgcGAGcUGGUGCUGCg -3' miRNA: 3'- -CGaGUAaaAGCU-------------UUCuACCGCGACGa -5' |
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| 32099 | 3' | -47.6 | NC_007016.1 | + | 12084 | 0.67 | 0.998587 |
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Target: 5'- -gUCAUaagCGAacugAAGcuGUGGCGCUGCg -3' miRNA: 3'- cgAGUAaaaGCU----UUC--UACCGCGACGa -5' |
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| 32099 | 3' | -47.6 | NC_007016.1 | + | 126022 | 0.67 | 0.997512 |
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Target: 5'- --------cCGGGAGcUGGCGCUGCa -3' miRNA: 3'- cgaguaaaaGCUUUCuACCGCGACGa -5' |
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| 32099 | 3' | -47.6 | NC_007016.1 | + | 113304 | 0.71 | 0.97259 |
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Target: 5'- uGCUCAUUgagcccugUUG-AAGAacUGGCGUUGCa -3' miRNA: 3'- -CGAGUAAa-------AGCuUUCU--ACCGCGACGa -5' |
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| 32099 | 3' | -47.6 | NC_007016.1 | + | 52154 | 0.71 | 0.965844 |
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Target: 5'- uCUCAga--UGAgccuaugguacagGAGAUGGCGCUGCUu -3' miRNA: 3'- cGAGUaaaaGCU-------------UUCUACCGCGACGA- -5' |
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| 32099 | 3' | -47.6 | NC_007016.1 | + | 52362 | 1.12 | 0.010182 |
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Target: 5'- gGCUCAUUUUCGAAAGAUGGCGCUGCUg -3' miRNA: 3'- -CGAGUAAAAGCUUUCUACCGCGACGA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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