Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32099 | 5' | -53.4 | NC_007016.1 | + | 59024 | 0.67 | 0.900722 |
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Target: 5'- --cGCGGUa--AUCUCCUGUGCCAg- -3' miRNA: 3'- guuCGUCGcggUAGAGGACAUGGUau -5' |
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| 32099 | 5' | -53.4 | NC_007016.1 | + | 67314 | 0.67 | 0.893894 |
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Target: 5'- gGGGCguGGCGUCGUCUCauaUGUugCAg- -3' miRNA: 3'- gUUCG--UCGCGGUAGAGg--ACAugGUau -5' |
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| 32099 | 5' | -53.4 | NC_007016.1 | + | 29117 | 0.68 | 0.864104 |
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Target: 5'- cCGGGCAGCaGUCcgCUC-UGUGCCAa- -3' miRNA: 3'- -GUUCGUCG-CGGuaGAGgACAUGGUau -5' |
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| 32099 | 5' | -53.4 | NC_007016.1 | + | 76114 | 0.68 | 0.864104 |
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Target: 5'- cCAGGCuua-CCAUCgCCUGUGCCAUGu -3' miRNA: 3'- -GUUCGucgcGGUAGaGGACAUGGUAU- -5' |
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| 32099 | 5' | -53.4 | NC_007016.1 | + | 93758 | 0.7 | 0.754773 |
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Target: 5'- gAGGUGGCGCCcaAUCUCCUaaaacuauacgGUGCCGa- -3' miRNA: 3'- gUUCGUCGCGG--UAGAGGA-----------CAUGGUau -5' |
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| 32099 | 5' | -53.4 | NC_007016.1 | + | 52204 | 0.73 | 0.57485 |
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Target: 5'- -cAGCAGCGCCAUCUuucgaaaaugagCCUG-GCCAa- -3' miRNA: 3'- guUCGUCGCGGUAGA------------GGACaUGGUau -5' |
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| 32099 | 5' | -53.4 | NC_007016.1 | + | 52324 | 1.07 | 0.004702 |
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Target: 5'- uCAAGCAGCGCCAUCUCCUGUACCAUAg -3' miRNA: 3'- -GUUCGUCGCGGUAGAGGACAUGGUAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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