Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 321 | 3' | -59.6 | AC_000010.1 | + | 9348 | 0.66 | 0.405872 |
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Target: 5'- gCCc-GUG-CGCCgCCGGcgUCGCCGCGuGCc -3' miRNA: 3'- -GGacCAUaGCGG-GGCU--AGCGGCGC-CG- -5' |
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| 321 | 3' | -59.6 | AC_000010.1 | + | 10674 | 0.68 | 0.316559 |
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Target: 5'- gCCUGGgaguggcUCGCgCCCGuagucuggagaaucaAUCGCCaggguugcguuGCGGCa -3' miRNA: 3'- -GGACCau-----AGCG-GGGC---------------UAGCGG-----------CGCCG- -5' |
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| 321 | 3' | -59.6 | AC_000010.1 | + | 17446 | 0.68 | 0.298511 |
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Target: 5'- gCUGGgggGUggcuuucugccUGCUCUGAUCcccaucauaGCCGCGGCg -3' miRNA: 3'- gGACCa--UA-----------GCGGGGCUAG---------CGGCGCCG- -5' |
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| 321 | 3' | -59.6 | AC_000010.1 | + | 27732 | 0.7 | 0.219596 |
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Target: 5'- cCCUGGUcgCG-CUCGAUUagcacggGCCGCuGGCa -3' miRNA: 3'- -GGACCAuaGCgGGGCUAG-------CGGCG-CCG- -5' |
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| 321 | 3' | -59.6 | AC_000010.1 | + | 8535 | 0.72 | 0.167121 |
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Target: 5'- uUCUGGUAcugCGCCCUGAgaagacuugcgugCGCgacaaCGCGGCg -3' miRNA: 3'- -GGACCAUa--GCGGGGCUa------------GCG-----GCGCCG- -5' |
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| 321 | 3' | -59.6 | AC_000010.1 | + | 17547 | 1.12 | 0.000149 |
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Target: 5'- gCCUGGUAUCGCCCCGAUCGCCGCGGCu -3' miRNA: 3'- -GGACCAUAGCGGGGCUAGCGGCGCCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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