Results 1 - 19 of 19 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
321 | 5' | -53.7 | AC_000010.1 | + | 17870 | 0.66 | 0.684005 |
Target: 5'- gGGAUCGccucugguauuaauGGGguGGUGGAucuGGCCAaCCa -3' miRNA: 3'- -CCUAGU--------------CUCguCCGUCUu--UCGGUgGG- -5' |
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321 | 5' | -53.7 | AC_000010.1 | + | 23345 | 0.66 | 0.676009 |
Target: 5'- aGAggCGGAGCugguGGCuguGGGcuucAGCUACCCa -3' miRNA: 3'- cCUa-GUCUCGu---CCGu--CUU----UCGGUGGG- -5' |
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321 | 5' | -53.7 | AC_000010.1 | + | 13598 | 0.66 | 0.676009 |
Target: 5'- gGGAUagAGAGCcuGGUGGAuaagauGAGCCGCUg -3' miRNA: 3'- -CCUAg-UCUCGu-CCGUCU------UUCGGUGGg -5' |
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321 | 5' | -53.7 | AC_000010.1 | + | 4867 | 0.66 | 0.676009 |
Target: 5'- --cUCGGAGCAGGgG---GGCCACUUc -3' miRNA: 3'- ccuAGUCUCGUCCgUcuuUCGGUGGG- -5' |
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321 | 5' | -53.7 | AC_000010.1 | + | 25425 | 0.66 | 0.653055 |
Target: 5'- aGGAUguGAGCGG--AGAuGGCCugCUu -3' miRNA: 3'- -CCUAguCUCGUCcgUCUuUCGGugGG- -5' |
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321 | 5' | -53.7 | AC_000010.1 | + | 12319 | 0.66 | 0.64154 |
Target: 5'- gGGGUUGGAGCGGG-AGGGcacuacGGCC-UCCa -3' miRNA: 3'- -CCUAGUCUCGUCCgUCUU------UCGGuGGG- -5' |
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321 | 5' | -53.7 | AC_000010.1 | + | 5333 | 0.66 | 0.64154 |
Target: 5'- cGAUCGGcAGCGGuucucgaccaGCAGGAugagccugaAGCgCACCCu -3' miRNA: 3'- cCUAGUC-UCGUC----------CGUCUU---------UCG-GUGGG- -5' |
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321 | 5' | -53.7 | AC_000010.1 | + | 18070 | 0.67 | 0.618493 |
Target: 5'- aGAUCGGGGC--GCGGAcgcuuGUCGCCCc -3' miRNA: 3'- cCUAGUCUCGucCGUCUuu---CGGUGGG- -5' |
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321 | 5' | -53.7 | AC_000010.1 | + | 32909 | 0.67 | 0.606984 |
Target: 5'- ---aCGGAGCGGcGCAGAcAGCgACUg -3' miRNA: 3'- ccuaGUCUCGUC-CGUCUuUCGgUGGg -5' |
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321 | 5' | -53.7 | AC_000010.1 | + | 35142 | 0.67 | 0.600089 |
Target: 5'- ---gCAGAGCAGaCAGGAGaacuauaaacugacuGCCGCCUg -3' miRNA: 3'- ccuaGUCUCGUCcGUCUUU---------------CGGUGGG- -5' |
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321 | 5' | -53.7 | AC_000010.1 | + | 15184 | 0.67 | 0.584047 |
Target: 5'- uGGAgCAGAGCAGGUguacuGGUCACUUc -3' miRNA: 3'- -CCUaGUCUCGUCCGucuu-UCGGUGGG- -5' |
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321 | 5' | -53.7 | AC_000010.1 | + | 32667 | 0.67 | 0.576057 |
Target: 5'- --uUCAGAGCGGGCcaaucuuGGAucagugauagauauaAAGCCAUCg -3' miRNA: 3'- ccuAGUCUCGUCCG-------UCU---------------UUCGGUGGg -5' |
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321 | 5' | -53.7 | AC_000010.1 | + | 22198 | 0.68 | 0.538774 |
Target: 5'- gGGAacuggCAGGuCAGGCAGGAuGUCGCuCCa -3' miRNA: 3'- -CCUa----GUCUcGUCCGUCUUuCGGUG-GG- -5' |
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321 | 5' | -53.7 | AC_000010.1 | + | 8592 | 0.68 | 0.516578 |
Target: 5'- uGGAUCuGucGCcucuGGguGAAAGCUACCg -3' miRNA: 3'- -CCUAGuCu-CGu---CCguCUUUCGGUGGg -5' |
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321 | 5' | -53.7 | AC_000010.1 | + | 16527 | 0.69 | 0.462849 |
Target: 5'- gGGAUgaugauauucUGGAGCAGGCGGcu-GaCCGCCUg -3' miRNA: 3'- -CCUA----------GUCUCGUCCGUCuuuC-GGUGGG- -5' |
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321 | 5' | -53.7 | AC_000010.1 | + | 26022 | 0.72 | 0.330223 |
Target: 5'- aGGAggCAGAGgAGGUGGAAGaagcaaccGCCGCCa -3' miRNA: 3'- -CCUa-GUCUCgUCCGUCUUU--------CGGUGGg -5' |
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321 | 5' | -53.7 | AC_000010.1 | + | 12934 | 0.72 | 0.313718 |
Target: 5'- uGGGUCAGgaAGaCAcuGGCAGcuugGAAGCCACCUu -3' miRNA: 3'- -CCUAGUC--UC-GU--CCGUC----UUUCGGUGGG- -5' |
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321 | 5' | -53.7 | AC_000010.1 | + | 15903 | 0.79 | 0.114402 |
Target: 5'- cGGA--AGAGCAGGCGGAGAcgcaucgccaggcGCCACCg -3' miRNA: 3'- -CCUagUCUCGUCCGUCUUU-------------CGGUGGg -5' |
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321 | 5' | -53.7 | AC_000010.1 | + | 17513 | 1.13 | 0.000382 |
Target: 5'- gGGAUCAGAGCAGGCAGAAAGCCACCCc -3' miRNA: 3'- -CCUAGUCUCGUCCGUCUUUCGGUGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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