Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32110 | 5' | -56.8 | NC_007016.1 | + | 17503 | 0.66 | 0.857323 |
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Target: 5'- gGUCgGUCUccccgGGGUgGUCUCGGCGGAg -3' miRNA: 3'- gCAGgUAGG-----UCUAgUGGAGCCGCCUa -5' |
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| 32110 | 5' | -56.8 | NC_007016.1 | + | 101564 | 0.66 | 0.849447 |
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Target: 5'- gCGUCCAccugUCUGGAUCAUCgcCaGCGGAUu -3' miRNA: 3'- -GCAGGU----AGGUCUAGUGGa-GcCGCCUA- -5' |
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| 32110 | 5' | -56.8 | NC_007016.1 | + | 118983 | 0.67 | 0.807226 |
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Target: 5'- -cUCC-UCCGGGaCGCCUgGGUGGAc -3' miRNA: 3'- gcAGGuAGGUCUaGUGGAgCCGCCUa -5' |
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| 32110 | 5' | -56.8 | NC_007016.1 | + | 20376 | 0.67 | 0.789156 |
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Target: 5'- cCGUacaCCGgccCCAGGUCGCCcucgCGGCGGu- -3' miRNA: 3'- -GCA---GGUa--GGUCUAGUGGa---GCCGCCua -5' |
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| 32110 | 5' | -56.8 | NC_007016.1 | + | 13711 | 0.68 | 0.761002 |
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Target: 5'- uGUCCGUCUGGAagcggcucggUACCUCGGCGc-- -3' miRNA: 3'- gCAGGUAGGUCUa---------GUGGAGCCGCcua -5' |
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| 32110 | 5' | -56.8 | NC_007016.1 | + | 130352 | 0.68 | 0.74164 |
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Target: 5'- ------cCCGGggCGCCUCGGCGGGg -3' miRNA: 3'- gcagguaGGUCuaGUGGAGCCGCCUa -5' |
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| 32110 | 5' | -56.8 | NC_007016.1 | + | 21817 | 0.7 | 0.649769 |
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Target: 5'- gCGUCCAUuaauacccgcggcCCGGGUcCGCCUCGaCGGAa -3' miRNA: 3'- -GCAGGUA-------------GGUCUA-GUGGAGCcGCCUa -5' |
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| 32110 | 5' | -56.8 | NC_007016.1 | + | 62539 | 0.7 | 0.64051 |
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Target: 5'- ---aCAUCUAGAUCACCggcagggGGCGGAUg -3' miRNA: 3'- gcagGUAGGUCUAGUGGag-----CCGCCUA- -5' |
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| 32110 | 5' | -56.8 | NC_007016.1 | + | 99746 | 1.06 | 0.003205 |
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Target: 5'- uCGUCCAUCCAGAUCACCUCGGCGGAUa -3' miRNA: 3'- -GCAGGUAGGUCUAGUGGAGCCGCCUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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