miRNA: 3'- uAGCCCGCA---CAAGaa-ACCU-AAGGCaa -5'
miRNA: 3'- uAGCCC-GCa--CAAGaaACCUAAGGCAa -5'
miRNA: 3'- uaGCCCG--CACaAGAAACCUAAGGCaa -5'
miRNA: 3'- -UAGCCCGCACAAGAAACCUAAGGCAA- -5'
Results 1 - 4 of 4 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32113 | 5' | -51.6 | NC_007016.1 | + | 27857 | 0.66 | 0.971451 |
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Target: 5'- cUCGGGCGUaagGUUCcggUGGAgUUCUGg- -3' miRNA: 3'- uAGCCCGCA---CAAGaa-ACCU-AAGGCaa -5' |
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| 32113 | 5' | -51.6 | NC_007016.1 | + | 60975 | 0.68 | 0.934752 |
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Target: 5'- cUUGGGuCGaacGUUCagUGGAUUCCGUg -3' miRNA: 3'- uAGCCC-GCa--CAAGaaACCUAAGGCAa -5' |
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| 32113 | 5' | -51.6 | NC_007016.1 | + | 112040 | 0.76 | 0.569296 |
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Target: 5'- -aCGGGCacGUG-UCUUUGGGUUCCGg- -3' miRNA: 3'- uaGCCCG--CACaAGAAACCUAAGGCaa -5' |
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| 32113 | 5' | -51.6 | NC_007016.1 | + | 112162 | 1.03 | 0.012883 |
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Target: 5'- gAUCGGGCGUGUUCUUUGGAUUCCGUUu -3' miRNA: 3'- -UAGCCCGCACAAGAAACCUAAGGCAA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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