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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32114 | 5' | -50.6 | NC_007016.1 | + | 55822 | 0.66 | 0.985805 |
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Target: 5'- -cCGAAUUuuucAgGGCGCUGCACaauaaGUUGu -3' miRNA: 3'- aaGCUUAAu---UgCCGCGACGUGc----CAAC- -5' |
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| 32114 | 5' | -50.6 | NC_007016.1 | + | 120288 | 0.66 | 0.985805 |
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Target: 5'- uUUCGGc--AGCGGCcgcgcGCUGCGCGGcgGa -3' miRNA: 3'- -AAGCUuaaUUGCCG-----CGACGUGCCaaC- -5' |
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| 32114 | 5' | -50.6 | NC_007016.1 | + | 124525 | 0.67 | 0.97964 |
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Target: 5'- -cUGAGUgacauuacgcUGACGGCGuCUGU-CGGUUGg -3' miRNA: 3'- aaGCUUA----------AUUGCCGC-GACGuGCCAAC- -5' |
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| 32114 | 5' | -50.6 | NC_007016.1 | + | 70714 | 0.68 | 0.957874 |
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Target: 5'- aUUCGAcgcaGUgcccgcACgGGCGCUGCGCGGg-- -3' miRNA: 3'- -AAGCU----UAau----UG-CCGCGACGUGCCaac -5' |
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| 32114 | 5' | -50.6 | NC_007016.1 | + | 116715 | 0.68 | 0.953808 |
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Target: 5'- -gCGAGgagGGCGGCGaugcGCACGGggGa -3' miRNA: 3'- aaGCUUaa-UUGCCGCga--CGUGCCaaC- -5' |
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| 32114 | 5' | -50.6 | NC_007016.1 | + | 13394 | 0.69 | 0.923904 |
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Target: 5'- cUUUGAA--AACGGCGC-GCGCGGa-- -3' miRNA: 3'- -AAGCUUaaUUGCCGCGaCGUGCCaac -5' |
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| 32114 | 5' | -50.6 | NC_007016.1 | + | 18387 | 0.73 | 0.779956 |
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Target: 5'- -aCGGuGUUGACGGCGCUGaGCuGGUUGu -3' miRNA: 3'- aaGCU-UAAUUGCCGCGACgUG-CCAAC- -5' |
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| 32114 | 5' | -50.6 | NC_007016.1 | + | 114605 | 0.98 | 0.03817 |
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Target: 5'- aUUCGAAUauACGGCGCUGCACGGUUGg -3' miRNA: 3'- -AAGCUUAauUGCCGCGACGUGCCAAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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