Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32115 | 3' | -48 | NC_007016.1 | + | 96259 | 0.66 | 0.999232 |
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Target: 5'- uGGGA-UCCCCgagGGCGuacGUGAAGUUAc -3' miRNA: 3'- -CCCUaAGGGGa--UUGUcc-CACUUUAAU- -5' |
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| 32115 | 3' | -48 | NC_007016.1 | + | 49291 | 0.67 | 0.99792 |
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Target: 5'- cGGGGcUUCCCC-AACguagGGGGUGGc---- -3' miRNA: 3'- -CCCU-AAGGGGaUUG----UCCCACUuuaau -5' |
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| 32115 | 3' | -48 | NC_007016.1 | + | 114572 | 0.68 | 0.994265 |
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Target: 5'- gGGGAUUCCCCaAACAaGGUa------ -3' miRNA: 3'- -CCCUAAGGGGaUUGUcCCAcuuuaau -5' |
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| 32115 | 3' | -48 | NC_007016.1 | + | 117178 | 0.68 | 0.992262 |
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Target: 5'- cGGGUUCCCCUGuuGGGGg------- -3' miRNA: 3'- cCCUAAGGGGAUugUCCCacuuuaau -5' |
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| 32115 | 3' | -48 | NC_007016.1 | + | 21965 | 0.7 | 0.980618 |
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Target: 5'- gGGGAUUCCCCaAACAGGu-------- -3' miRNA: 3'- -CCCUAAGGGGaUUGUCCcacuuuaau -5' |
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| 32115 | 3' | -48 | NC_007016.1 | + | 51217 | 0.71 | 0.969768 |
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Target: 5'- cGGGAgaaacaUCCCCUGAaccgcgGGGGUGAc---- -3' miRNA: 3'- -CCCUa-----AGGGGAUUg-----UCCCACUuuaau -5' |
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| 32115 | 3' | -48 | NC_007016.1 | + | 65623 | 0.71 | 0.966486 |
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Target: 5'- cGGAcauuaUUCCCCUuuuACAGGGagUGAGGUUu -3' miRNA: 3'- cCCU-----AAGGGGAu--UGUCCC--ACUUUAAu -5' |
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| 32115 | 3' | -48 | NC_007016.1 | + | 114791 | 0.77 | 0.73916 |
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Target: 5'- gGGGAUUCCCCUAACAaGGUa------ -3' miRNA: 3'- -CCCUAAGGGGAUUGUcCCAcuuuaau -5' |
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| 32115 | 3' | -48 | NC_007016.1 | + | 23223 | 0.82 | 0.50145 |
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Target: 5'- uGGGAUUCCCUUAACAGGGa------- -3' miRNA: 3'- -CCCUAAGGGGAUUGUCCCacuuuaau -5' |
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| 32115 | 3' | -48 | NC_007016.1 | + | 115131 | 1.1 | 0.012211 |
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Target: 5'- gGGGAUUCCCCUAACAGGGUGAAAUUAu -3' miRNA: 3'- -CCCUAAGGGGAUUGUCCCACUUUAAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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