miRNA: 3'- uAAUUAAUGuAGa--ACAAUCCcCCUAG- -5'
miRNA: 3'- -UAAUUaAUGUAGAACAAUCC-C-CCUag -5'
miRNA: 3'- uaaUUAAUGUAGaaCAAU---CCCCCUAg -5'
miRNA: 3'- -UAAUUAAUGUaGAACAAUCCCCcUAG- -5'
miRNA: 3'- -UAAUUAAUGUAGAACAAUCCCCCUAG- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32115 | 5' | -43.2 | NC_007016.1 | + | 30439 | 0.68 | 0.999983 |
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Target: 5'- uUUGGUUGCuUCgcgUGUUGGGcGGAUUg -3' miRNA: 3'- uAAUUAAUGuAGa--ACAAUCCcCCUAG- -5' |
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| 32115 | 5' | -43.2 | NC_007016.1 | + | 45004 | 0.69 | 0.999887 |
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Target: 5'- gAUUAAaUGCAUUUUGUUAGGcGuGGAa- -3' miRNA: 3'- -UAAUUaAUGUAGAACAAUCC-C-CCUag -5' |
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| 32115 | 5' | -43.2 | NC_007016.1 | + | 121016 | 0.72 | 0.997141 |
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Target: 5'- --aAGUUGCGUCggGUUAauuGGGGGGUa -3' miRNA: 3'- uaaUUAAUGUAGaaCAAU---CCCCCUAg -5' |
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| 32115 | 5' | -43.2 | NC_007016.1 | + | 114951 | 0.78 | 0.94196 |
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Target: 5'- cAUUAGUUAUAcCUUGUUAGGGGaAUCc -3' miRNA: 3'- -UAAUUAAUGUaGAACAAUCCCCcUAG- -5' |
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| 32115 | 5' | -43.2 | NC_007016.1 | + | 115094 | 1.08 | 0.044272 |
|
Target: 5'- uAUUAAUUACAUCUUGUUAGGGGGAUCc -3' miRNA: 3'- -UAAUUAAUGUAGAACAAUCCCCCUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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