Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
32117 | 3' | -51.2 | NC_007016.1 | + | 30243 | 0.66 | 0.974325 |
Target: 5'- cGGAAUcugucaguugGUGUaaGCCCUGcCAUCGCUGu -3' miRNA: 3'- cUCUUA----------UACAa-CGGGACuGUGGCGAC- -5' |
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32117 | 3' | -51.2 | NC_007016.1 | + | 91174 | 0.67 | 0.968354 |
Target: 5'- ---------aUGCgCUGGCGCCGCUGa -3' miRNA: 3'- cucuuauacaACGgGACUGUGGCGAC- -5' |
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32117 | 3' | -51.2 | NC_007016.1 | + | 54851 | 0.67 | 0.957646 |
Target: 5'- cGGGAAUGUGgcgGUCagUGGCacuACCGCUGa -3' miRNA: 3'- -CUCUUAUACaa-CGGg-ACUG---UGGCGAC- -5' |
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32117 | 3' | -51.2 | NC_007016.1 | + | 91095 | 0.69 | 0.929384 |
Target: 5'- uGAGGAcg-GggGCCCUGcCcugGCCGCUGa -3' miRNA: 3'- -CUCUUauaCaaCGGGACuG---UGGCGAC- -5' |
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32117 | 3' | -51.2 | NC_007016.1 | + | 121427 | 0.7 | 0.898555 |
Target: 5'- aGAGAGUcgggcgAUGUUGCCgUGGCGgCGuCUGc -3' miRNA: 3'- -CUCUUA------UACAACGGgACUGUgGC-GAC- -5' |
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32117 | 3' | -51.2 | NC_007016.1 | + | 116116 | 0.87 | 0.158233 |
Target: 5'- gGAGAAUAUGUU-CCgCUGACACCGCUGc -3' miRNA: 3'- -CUCUUAUACAAcGG-GACUGUGGCGAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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