Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
32121 | 3' | -50.8 | NC_007016.1 | + | 17621 | 0.68 | 0.963252 |
Target: 5'- cGCGUCaAUGgagagcgcccCGGUGUCUGGUGAc-- -3' miRNA: 3'- -CGCAGgUACa---------GCCACAGACCGUUuuc -5' |
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32121 | 3' | -50.8 | NC_007016.1 | + | 57159 | 0.69 | 0.955719 |
Target: 5'- uGCGU-UGUGaaGGUGUCUGGUAAAGa -3' miRNA: 3'- -CGCAgGUACagCCACAGACCGUUUUc -5' |
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32121 | 3' | -50.8 | NC_007016.1 | + | 77351 | 0.69 | 0.937666 |
Target: 5'- cGCG-CCAUGguaGG-GUCUGGUGAAAa -3' miRNA: 3'- -CGCaGGUACag-CCaCAGACCGUUUUc -5' |
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32121 | 3' | -50.8 | NC_007016.1 | + | 37837 | 0.7 | 0.932512 |
Target: 5'- cGCGUgCCGUGcgcucCGGcGUCUGGUAuuGGg -3' miRNA: 3'- -CGCA-GGUACa----GCCaCAGACCGUuuUC- -5' |
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32121 | 3' | -50.8 | NC_007016.1 | + | 121645 | 1.12 | 0.004921 |
Target: 5'- gGCGUCCAUGUCGGUGUCUGGCAAAAGg -3' miRNA: 3'- -CGCAGGUACAGCCACAGACCGUUUUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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