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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
32166 | 5' | -50.3 | NC_007016.1 | + | 39802 | 0.67 | 0.986313 |
Target: 5'- uGUCUUUGCCAauucucUUCUCGUCguacuggGGUGUUa -3' miRNA: 3'- gUAGAAGCGGU------GAGAGUAG-------UCACAGg -5' |
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32166 | 5' | -50.3 | NC_007016.1 | + | 79209 | 0.68 | 0.966889 |
Target: 5'- uCAUCUUCaCCGCUCgugcccGUGUCCg -3' miRNA: 3'- -GUAGAAGcGGUGAGaguaguCACAGG- -5' |
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32166 | 5' | -50.3 | NC_007016.1 | + | 62453 | 1.12 | 0.005382 |
Target: 5'- gCAUCUUCGCCACUCUCAUCAGUGUCCu -3' miRNA: 3'- -GUAGAAGCGGUGAGAGUAGUCACAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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