Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32175 | 3' | -57.3 | NC_007016.1 | + | 108583 | 0.66 | 0.814514 |
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Target: 5'- cGUGGCgcccccugccuGGGUGCGGAGCAaagcguGCuuGuGCGa -3' miRNA: 3'- -CACUG-----------CCCAUGCUUCGU------CGggCuCGU- -5' |
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| 32175 | 3' | -57.3 | NC_007016.1 | + | 123127 | 0.66 | 0.796689 |
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Target: 5'- cGUGAgGGGUuucuCuGAGUAGaCCGAGCGc -3' miRNA: 3'- -CACUgCCCAu---GcUUCGUCgGGCUCGU- -5' |
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| 32175 | 3' | -57.3 | NC_007016.1 | + | 9933 | 0.67 | 0.776389 |
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Target: 5'- aUGGgGGGgcgucgcauacgGCGAGGC-GUCCGAGCu -3' miRNA: 3'- cACUgCCCa-----------UGCUUCGuCGGGCUCGu -5' |
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| 32175 | 3' | -57.3 | NC_007016.1 | + | 114129 | 0.67 | 0.759305 |
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Target: 5'- gGUGGCuccGGGUGgGGuGGCGGCuCCGGGUg -3' miRNA: 3'- -CACUG---CCCAUgCU-UCGUCG-GGCUCGu -5' |
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| 32175 | 3' | -57.3 | NC_007016.1 | + | 114185 | 0.67 | 0.759305 |
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Target: 5'- gGUGGCuccGGGUGgGGuGGCGGCuCCGGGUg -3' miRNA: 3'- -CACUG---CCCAUgCU-UCGUCG-GGCUCGu -5' |
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| 32175 | 3' | -57.3 | NC_007016.1 | + | 13879 | 0.71 | 0.556702 |
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Target: 5'- cGUG-CGGGUGCGAGGU-GUUCGAGUc -3' miRNA: 3'- -CACuGCCCAUGCUUCGuCGGGCUCGu -5' |
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| 32175 | 3' | -57.3 | NC_007016.1 | + | 935 | 0.72 | 0.49727 |
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Target: 5'- -cGGCGGGgcccgGCGGAGCGGgaCCCGGGg- -3' miRNA: 3'- caCUGCCCa----UGCUUCGUC--GGGCUCgu -5' |
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| 32175 | 3' | -57.3 | NC_007016.1 | + | 28536 | 0.74 | 0.363547 |
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Target: 5'- gGUGAUGGGUGCGAgaggauGGCGGCCa-GGCc -3' miRNA: 3'- -CACUGCCCAUGCU------UCGUCGGgcUCGu -5' |
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| 32175 | 3' | -57.3 | NC_007016.1 | + | 78495 | 1.09 | 0.001815 |
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Target: 5'- cGUGACGGGUACGAAGCAGCCCGAGCAg -3' miRNA: 3'- -CACUGCCCAUGCUUCGUCGGGCUCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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